This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Stabilization and destabilization of mRNA plays an important role in the regulation of gene expression. Our knowledge of presence of human mRNA and the stabilization that enables its presence in human saliva is very poor. The existence of viral, bacterial and human RNAs in saliva allows us to study the mechanisms and regulations of mRNA stability for various diseases. The challenges related to regulation of mRNA stability in human saliva, and how mRNA is stabilized by utilizing mRNA stability pathways is yet to uncover. We hypothesize that there are sequence elements and protein factors responsible for the differential presence of disease specific RNA biomarkers in saliva or oral cancer patients. This research will allow us to gain insights into the sequence elements and protein factors responsible for mRNA stability in human saliva. Therefore, the major goal of this proposal is to understand the mechanistic aspects of mRNA stability in human saliva.
Two specific aims are proposed to accomplish these goals. I) Determine the fundamental mechanisms responsible for the stabilization of mRNA's in human saliva and II) Validate the AU-rich element containing mRNA decay in correlation with MAP kinase pathway activation.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR017696-09
Application #
8167773
Study Section
Special Emphasis Panel (ZRR1-RI-5 (01))
Project Start
2010-06-01
Project End
2011-05-31
Budget Start
2010-06-01
Budget End
2011-05-31
Support Year
9
Fiscal Year
2010
Total Cost
$213,141
Indirect Cost
Name
Medical University of South Carolina
Department
Microbiology/Immun/Virology
Type
Schools of Dentistry
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
Yuen, Hon K (2018) Factors associated with additional time dental hygienists spent on educating patients with diabetes. Spec Care Dentist 38:313-318
Heise, Tilman; Kota, Venkatesh; Brock, Alexander et al. (2016) The La protein counteracts cisplatin-induced cell death by stimulating protein synthesis of anti-apoptotic factor Bcl2. Oncotarget 7:29664-76
Sabatini, Camila; Mennito, Anthony S; Wolf, Bethany J et al. (2015) Incorporation of bactericidal poly-acrylic acid modified copper iodide particles into adhesive resins. J Dent 43:546-55
Wood, James S; Marlow, Nicole M; Cayouette, Monica J (2015) Accuracy of dental torque wrenches. Gen Dent 63:e20-2
Hunt, Kelly J; Kistner-Griffin, Emily; Spruill, Ida et al. (2014) Cardiovascular risk in Gullah African Americans with high familial risk of type 2 diabetes mellitus: project SuGAR. South Med J 107:607-14
Yuen, H K; Weng, Y; Reed, S G et al. (2014) Factors associated with gingival inflammation among adults with systemic sclerosis. Int J Dent Hyg 12:55-61
Cooley, Marion A; Harikrishnan, Keerthi; Oppel, James A et al. (2014) Fibulin-1 is required for bone formation and Bmp-2-mediated induction of Osterix. Bone 69:30-8
Cantini, Liliana P; Andino, Lourdes M; Attaway, Christopher C et al. (2014) Identification and characterization of Dicer1e, a Dicer1 protein variant, in oral cancer cells. Mol Cancer 13:190
Shi, Changcheng; Cisewski, Sarah E; Bell, P Darwin et al. (2014) Measurement of three-dimensional anisotropic diffusion by multiphoton fluorescence recovery after photobleaching. Ann Biomed Eng 42:555-65
Qin, Tingting; Matmati, Nabil; Tsoi, Lam C et al. (2014) Finding pathway-modulating genes from a novel Ontology Fingerprint-derived gene network. Nucleic Acids Res 42:e138

Showing the most recent 10 out of 136 publications