This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core facility supports the research of the COBRE investigators. Specifically, high-resolution electrospray-quadrupole/time-of-flight mass spectrometer is being used in Project 2 (Porter) for identification of pro-inflammatory arachidonic acid metabolites generated following stimulation of neuroinflammatory calcitonin gene-related peptide receptor, and to quantitate the amount of prostaglandin compounds hypothesized to be produced from an increased expression of cyclooxygenase-2. In Project 3 (Picklo), mass spectrometry is being used to study mechanisms of 4-hydroxy-2(E)-nonenal (HNE) methabolism in the central nervous system tissue, electrospray-triple quadrupole mass spectrometer will be used to identify and quantitate HNE methabolites, and electrospray-quadrupole/time-of-flight mass spectrometer will be used to identify novel detoxification enzymes. In Project 4 (Murphy), electrospray-triple quadrupole and gas chromatography-ion trap mass spectrometer is being used to measure phospholipids head group flux and turnover in neuronal, astroglia, and stably transfected HEK-293 cell lines, and to measure labeled and unlabeled phospholipids to study the kinetics of compound incorporation. The core facility consists of three instruments; electrospray-quadrupole/time-of-flight, electrospray-triple quadrupole and gas chromatography-ion trap mass spectrometer. These instruments provide us a capability of determining chemical structures of a wide variety of compounds generated in this COBRE as well as a capability of quantifying the compounds. The facility responsibilities (training users and helping with data analysis, operation and maintenance, assuring that the standard operating procedures are followed) will be divided between the facility director and the research assistant.
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