This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator. The Core Proteomics Laboratory is uniquely equipped with robotic workstations that increase gel throughput, image analysis, spot excision, in-gel peptide digestion, and MALDI-MS sample preparation. The automated workstations include a Propic gel spot picker that visualizes and excises spots from fluorescence-stained gels and a ProGest station that automates in-gel trypsin digestion in a 96- or 384-well format. The laboratory also contains a ProMS robot that automates desalting, concentrating and spotting of peptide samples onto MALDI targets, allowing fully integrated high-throughput analysis of protein samples. MALDI-MS analysis is performed using a Micromass Inc. TOF-Spec 2E. The lab recently instituted automated analysis of high-resolution 2D gels using Progenesis software, significantly decreasing analysis time of 2D gels, accelerating throughput. The CPL maintains active collaborations and provides instruction to COBRE-affiliated faculty and graduate students.
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