This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.This core focuses on identifying the nature of cells in skin and skeletal muscle after marrow cells transplantation. The overall plan has been for donor cells and their specific lineages to be ascertained and colocalized by confocal and deconvolution fluorescent microscopy. In this plan, donor cells can be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of make DNA. The lineage of identified donor cells can be ascertained by standard morphology, geographical location and immunohistochemistry. Colocalization of donor and lineage markers canbe confirmed using confocal or deconvolution fluorescent microscopy. The core canprovide high-level expertise for these approaches. The core contains two Zeiss fluorescent motor-driven microscopes, an upright Axioplan 2 and an inverted Axiovert 200M, and a Zeiss 510 confocal laser-scanning microscope capable of four-color imaging. The core is capable of standard deconvolution fluorescent microscopy, 3D reconstructions and confocal microscopy. These latter, plus photomapping can be used to simulateously show a donor marker and a cell type specific marker.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR018757-05
Application #
7610572
Study Section
Special Emphasis Panel (ZRR1-RI-3 (01))
Project Start
2007-05-01
Project End
2008-04-30
Budget Start
2007-05-01
Budget End
2008-04-30
Support Year
5
Fiscal Year
2007
Total Cost
$137,203
Indirect Cost
Name
Roger Williams Hospital
Department
Type
DUNS #
625899281
City
Providence
State
RI
Country
United States
Zip Code
02908
Kim, Joseph W; Vang, Souriya; Luo, John Zq et al. (2017) Effects of bone marrow on the microenvironment of the human pancreatic islet: A Protein Profile Approach. Mol Cell Endocrinol 450:32-42
Tang, Jin Bo; Wu, Ya Fang; Cao, Yi et al. (2016) Basic FGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons. Sci Rep 6:20643
Luo, John Z Q; Kim, Joseph W; Luo, LuGuang (2016) EFFECTS OF GINSENG AND ITS FOUR PURIFED GINSENOSIDES (Rb2, Re, Rg1, Rd) ON HUMAN PANCREATIC ISLET ? CELL IN VITRO. Eur J Pharm Med Res 3:110-119
Kim, Joseph W; Luo, John Z; Luo, Luguang (2015) The Biochemical Cascades of the Human Pancreatic ?-Cells: The Role of MicroRNAs. J Bioanal Biomed 7:
Luo, Lu Guang; Xiong, Fang; Ravassard, Philippe et al. (2015) Human Bone Marrow Subpopulations Sustain Human Islet Function and Viability In vitro. Br J Med Med Res 8:576-587
Ilgun, Handenur; Kim, Joseph William; Luo, LuGuang (2015) Adult Stem Cells and Diabetes Therapy. J Stem Cell Res Transplant 2:
Bartos, Adrian; Dubielecka, Patrycja M (2014) The emerging role of Bcr-Abl-induced cystoskeletal remodeling in systemic persistence of leukemic stem cells. Curr Drug Deliv 11:582-91
Chorzalska, A; Dubielecka, P M (2014) New Abelson interactor-1 (Abi-1)-driven mechanism of acquired drug resistance. Leuk Suppl 3:S7-8
Chorzalska, A; Salloum, I; Shafqat, H et al. (2014) Low expression of Abelson interactor-1 is linked to acquired drug resistance in Bcr-Abl-induced leukemia. Leukemia 28:2165-77
Dabiri, Ganary; Falanga, Vincent (2013) Connective tissue ulcers. J Tissue Viability 22:92-102

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