This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The Cell Sorter Core provides expertise in marrow or skin stem cell separations and analysis of donor cells in cell suspensions. John Morgan is the full time stem cell/sorter technician. This core features a MoFIo highspeed cell sorter from Cytomation. This sorter is capable of carrying out stem cell separations, single cell deposition and large event analysis. The FACS facility is also equipped with a FACS scan and a FACS calibur (BD instruments). Both machines can act as a backup for any analysis work required by the projects. Investigators work with the core and prepare their cell populations for sorting or analysis. The core personnel routinely separate Lin- Rho(lo)Ho(lo), Lin - Hoechst side populations and Lin - Sca+ CD45- cells and analyze populations for GFP+ or CFDA-SE positive cells. The core serves all projects in this COBRE carrying out both separations and analysis work. The core also remains dedicated to the development of new and the enhancement of already known stem cell separations.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
2P20RR018757-06
Application #
7725250
Study Section
Special Emphasis Panel (ZRR1-RI-6 (01))
Project Start
2008-06-05
Project End
2009-04-30
Budget Start
2008-06-05
Budget End
2009-04-30
Support Year
6
Fiscal Year
2008
Total Cost
$269,753
Indirect Cost
Name
Roger Williams Hospital
Department
Type
DUNS #
625899281
City
Providence
State
RI
Country
United States
Zip Code
02908
Kim, Joseph W; Vang, Souriya; Luo, John Zq et al. (2017) Effects of bone marrow on the microenvironment of the human pancreatic islet: A Protein Profile Approach. Mol Cell Endocrinol 450:32-42
Luo, John Z Q; Kim, Joseph W; Luo, LuGuang (2016) EFFECTS OF GINSENG AND ITS FOUR PURIFED GINSENOSIDES (Rb2, Re, Rg1, Rd) ON HUMAN PANCREATIC ISLET ? CELL IN VITRO. Eur J Pharm Med Res 3:110-119
Tang, Jin Bo; Wu, Ya Fang; Cao, Yi et al. (2016) Basic FGF or VEGF gene therapy corrects insufficiency in the intrinsic healing capacity of tendons. Sci Rep 6:20643
Kim, Joseph W; Luo, John Z; Luo, Luguang (2015) The Biochemical Cascades of the Human Pancreatic ?-Cells: The Role of MicroRNAs. J Bioanal Biomed 7:
Luo, Lu Guang; Xiong, Fang; Ravassard, Philippe et al. (2015) Human Bone Marrow Subpopulations Sustain Human Islet Function and Viability In vitro. Br J Med Med Res 8:576-587
Ilgun, Handenur; Kim, Joseph William; Luo, LuGuang (2015) Adult Stem Cells and Diabetes Therapy. J Stem Cell Res Transplant 2:
Bartos, Adrian; Dubielecka, Patrycja M (2014) The emerging role of Bcr-Abl-induced cystoskeletal remodeling in systemic persistence of leukemic stem cells. Curr Drug Deliv 11:582-91
Chorzalska, A; Dubielecka, P M (2014) New Abelson interactor-1 (Abi-1)-driven mechanism of acquired drug resistance. Leuk Suppl 3:S7-8
Chorzalska, A; Salloum, I; Shafqat, H et al. (2014) Low expression of Abelson interactor-1 is linked to acquired drug resistance in Bcr-Abl-induced leukemia. Leukemia 28:2165-77
Dabiri, Ganary; Falanga, Vincent (2013) Connective tissue ulcers. J Tissue Viability 22:92-102

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