This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The development of the sensory and motor components of the peripheral nervous system (PNS) is tightly regulated by a complex network of signaling molecules. These signaling molecules control cellular processes such as cell migration, proliferation, differentiation, fate specification, axon guidance, and the establishment of specific synapses. In the central nervous system, signal transduction through members of the erbB family, including the epidermal growth factor receptor (EGFR), influences glial and neuronal proliferation, differentiation, fate acquisition, neurite outgrowth, and survival. Loss of EGFR results in defective neuronal migration in the CNS as well as abnormal proliferation, differentiation, and maintenance of the cutaneous epithelium. Signal transduction through EGFR family members, all of which are expressed in the PNS, is vital for normal development of both motor and sensory neurons. This proposal is designed to test the hypotheses that EGFR signaling is also required for proper development of the peripheral nervous system and that interactions between neural and target cells regulate PNS development.
Specific aim 1. To determine the role of EGFR in the development of the PNS by examining the effects of loss of EGFR on innervation of the skin during development using EGFR homozygous null mice.
Specific aim 2. To determine whether developmental regulation of the PNS by EGFR is cell autonomous or regulated by the expression of EGFR in the target tissue.
Specific Aim 3. To identify downstream effector genes through which EGFR regulates the development of the PNS.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR018788-05
Application #
7610614
Study Section
Special Emphasis Panel (ZRR1-RI-3 (01))
Project Start
2007-05-01
Project End
2008-04-30
Budget Start
2007-05-01
Budget End
2008-04-30
Support Year
5
Fiscal Year
2007
Total Cost
$296,767
Indirect Cost
Name
University of Nebraska Medical Center
Department
Pediatrics
Type
Schools of Medicine
DUNS #
168559177
City
Omaha
State
NE
Country
United States
Zip Code
68198
Barta, Cody L; Liu, Huizhan; Chen, Lei et al. (2018) RNA-seq transcriptomic analysis of adult zebrafish inner ear hair cells. Sci Data 5:180005
Sanchez, Sonia M Rocha; Fuson, Olivia; Tarang, Shikha et al. (2018) Quinoxaline protects zebrafish lateral line hair cells from cisplatin and aminoglycosides damage. Sci Rep 8:15119
Liu, Huizhan; Chen, Lei; Giffen, Kimberlee P et al. (2018) Cell-Specific Transcriptome Analysis Shows That Adult Pillar and Deiters' Cells Express Genes Encoding Machinery for Specializations of Cochlear Hair Cells. Front Mol Neurosci 11:356
Bouska, A; Zhang, W; Gong, Q et al. (2017) Combined copy number and mutation analysis identifies oncogenic pathways associated with transformation of follicular lymphoma. Leukemia 31:83-91
Donze-Reiner, Teresa; Palmer, Nathan A; Scully, Erin D et al. (2017) Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugs. BMC Plant Biol 17:46
Gong, Qiang; Wang, Chao; Zhang, Weiwei et al. (2017) Assessment of T-cell receptor repertoire and clonal expansion in peripheral T-cell lymphoma using RNA-seq data. Sci Rep 7:11301
Olivares, A M; Jelcick, A S; Reinecke, J et al. (2017) Multimodal Regulation Orchestrates Normal and Complex Disease States in the Retina. Sci Rep 7:690
Goodman, Linda; Zallocchi, Marisa (2017) Integrin ?8 and Pcdh15 act as a complex to regulate cilia biogenesis in sensory cells. J Cell Sci 130:3698-3712
Amaradasa, Bimal S; Amundsen, Keenan (2016) Transcriptome Profiling of Buffalograss Challenged with the Leaf Spot Pathogen Curvularia inaequalis. Front Plant Sci 7:715
Rohr, J; Guo, S; Huo, J et al. (2016) Recurrent activating mutations of CD28 in peripheral T-cell lymphomas. Leukemia 30:1062-70

Showing the most recent 10 out of 92 publications