This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.One overall function of this core is to provide state-of-the-art capabilities for the analysis and separation of cell populations based on physical, immunological, genetic, and other characteristics. These capabilities are of particular importance in working with stem and progenitor cell populations, but also find application in nearly all other types of cell biology research. The Core is equipped with dedicated instruments for the analysis, isolation or depletion of most cell types. These include a Becton Dickinson FACSCalibur flow cytometer, a new FACSAria cell sorter, and a Miltenyi MACS Sorter (Automated Magnetic Cell Sorter). Users are Institute wide, and include investigators in both COBREs (COBRE Vascular Biology and COBRE Stem Cell Biology and Regenerative Medicine). In addition, this core is being developed as a resource for the study of embryonic stem (ES) cells. Technical support, training, and services for stem cell research are provided (especially by Zack Wang). Mouse and NIH-approved human ES cell lines will be maintained with appropriate quality assurance. Technical support provided by the facility will include hands-on instruction in the maintenance, transduction, and differentiation of ES cells. The facility also will provide short-term culture and manipulation of ES cells for individual investigators, and will provide ready access to ES cell reagents (RNAs, genomic DNAs, proteins) without the need to develop specialized technical skills (or space) for ES cell work.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR018789-05
Application #
7610630
Study Section
Special Emphasis Panel (ZRR1-RI-3 (01))
Project Start
2007-07-01
Project End
2008-06-30
Budget Start
2007-07-01
Budget End
2008-06-30
Support Year
5
Fiscal Year
2007
Total Cost
$173,630
Indirect Cost
Name
Maine Medical Center
Department
Type
DUNS #
071732663
City
Portland
State
ME
Country
United States
Zip Code
04102
Duarte, Christine W; Black, Adam W; Lucas, F Lee et al. (2017) Cancer incidence in patients with hereditary hemorrhagic telangiectasia. J Cancer Res Clin Oncol 143:209-214
Caron, Jennifer M; Ames, Jacquelyn J; Contois, Liangru et al. (2016) Inhibition of Ovarian Tumor Growth by Targeting the HU177 Cryptic Collagen Epitope. Am J Pathol 186:1649-61
Stohn, J Patrizia; Wang, Qiaozeng; Siviski, Matthew E et al. (2015) Cthrc1 controls adipose tissue formation, body composition, and physical activity. Obesity (Silver Spring) 23:1633-42
Ufkin, Melanie L; Peterson, Sarah; Yang, Xuehui et al. (2014) miR-125a regulates cell cycle, proliferation, and apoptosis by targeting the ErbB pathway in acute myeloid leukemia. Leuk Res 38:402-10
He, Qing; Yang, Xuehui; Gong, Yan et al. (2014) Deficiency of Sef is associated with increased postnatal cortical bone mass by regulating Runx2 activity. J Bone Miner Res 29:1217-31
Motyl, Katherine J; Bishop, Kathleen A; DeMambro, Victoria E et al. (2013) Altered thermogenesis and impaired bone remodeling in Misty mice. J Bone Miner Res 28:1885-97
Yoon, Jeong Kyo; Lee, Jin-Seon (2012) Cellular signaling and biological functions of R-spondins. Cell Signal 24:369-77
Favreau, Amanda J; Sathyanarayana, Pradeep (2012) miR-590-5p, miR-219-5p, miR-15b and miR-628-5p are commonly regulated by IL-3, GM-CSF and G-CSF in acute myeloid leukemia. Leuk Res 36:334-41
Urs, Sumithra; Henderson, Terry; Le, Phuong et al. (2012) Tissue-specific expression of Sprouty1 in mice protects against high-fat diet-induced fat accumulation, bone loss and metabolic dysfunction. Br J Nutr 108:1025-33
Sathyanarayana, Pradeep; Dev, Arvind; Pradeep, Anamika et al. (2012) Spry1 as a novel regulator of erythropoiesis, EPO/EPOR target, and suppressor of JAK2. Blood 119:5522-31

Showing the most recent 10 out of 102 publications