This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The hallmark of the vertebrate immune system is its ability to maintain an equilibrium between reactivity and quiescence of responses, while geared to respond and adapt rapidly to noxious and potentially lethal challenge. The system must recognize the challenge, develop and express a response to control the challenge, and terminate the reaction in regulating the response. These reactions are the responsibility of components of innate immunity that include cellular receptors and biomolecules at mucosal surfaces and within the systemic circulation. Due to the complexity of the microbial challenge in the oral cavity, it is clear that the innate immune system must play a crucial role in regulating host responses to the commensal microbiota. This proposal engages a collaborative relationship to enable determination of these responses in a human-like model of oral infection and inflammation: (1) This proposal will focus on the use of a nonhuman primate (rhesus) model of periodontal infection and inflammation. It is built upon a unique resource, the Caribbean Primate Research Center (CPRC);(2) This proposal is a collaborative relationship between investigators at the University of Kentucky, the CPRC, and the University of Puerto Rico Dental School;(3) This proposal will provide novel information on innate immunity in cross-sectional studies related to age using various cohorts from young to aged animals, and related to the expression of periodontitis;and, (4) Using the nonhuman primate model of periodontal infection and disease, we will determine longitudinal changes in innate immune responses througout age of development of the individual, specifically related to the initiation, progression, and resolution of the disease process. This model provides for a controlled study of these processes that cannot be accomplished in humans, but provides a human-like model of infection, disease and host responses. The General Hypothesis for the project is that """"""""Innate immune response components in gingival tissues vary with age and periodontal disease expression."""""""" This will enable the identification of crucial innate immune molecular events that contribute to gingival homeostasis. The studies proposed in this application will use an innovative strategy in 2 Specific Aims to interface a nonhuman primate model of periodontitis, with microarray gene expression technology to address these critical questions regarding the characteristics and ontogeny of innate responses in the oral cavity.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR020145-07
Application #
8168485
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Project Start
2010-08-01
Project End
2011-07-31
Budget Start
2010-08-01
Budget End
2011-07-31
Support Year
7
Fiscal Year
2010
Total Cost
$307,474
Indirect Cost
Name
University of Kentucky
Department
Dentistry
Type
Schools of Dentistry
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40506
Gonzalez, O A; Kirakodu, S; Novak, M J et al. (2018) Comparative analysis of microbial sensing molecules in mucosal tissues with aging. Immunobiology 223:279-287
Ebersole, J L; Dawson 3rd, D A; Emecen Huja, P et al. (2018) Age and Periodontal Health - Immunological View. Curr Oral Health Rep 5:229-241
Danaher, Robert J; Zhang, Liping; Donley, Connor J et al. (2018) Histone deacetylase inhibitors prevent persistent hypersensitivity in an orofacial neuropathic pain model. Mol Pain 14:1744806918796763
Ebersole, Jeffrey L; Orraca, Luis; Kensler, Terry B et al. (2018) Periodontal disease susceptible matrilines in the Cayo Santiago Macaca mulatta macaques. J Periodontal Res :
Ebersole, J L; Kirakodu, S; Novak, M J et al. (2018) Comparative analysis of expression of microbial sensing molecules in mucosal tissues with periodontal disease. Immunobiology :
Ebersole, Jeffrey L; Al-Sabbagh, Mohanad; Gonzalez, Octavio A et al. (2018) Ageing effects on humoral immune responses in chronic periodontitis. J Clin Periodontol 45:680-692
Lyons, Danielle N; Zhang, Liping; Pandya, Jignesh D et al. (2018) Combination Drug Therapy of Pioglitazone and D-cycloserine Attenuates Chronic Orofacial Neuropathic Pain and Anxiety by Improving Mitochondrial Function Following Trigeminal Nerve Injury. Clin J Pain 34:168-177
Lyons, Danielle N; Zhang, Liping; Danaher, Robert J et al. (2017) PPAR? Agonists Attenuate Trigeminal Neuropathic Pain. Clin J Pain 33:1071-1080
Nagarajan, R; Miller, C S; Dawson 3rd, D et al. (2017) Cross-talk between clinical and host-response parameters of periodontitis in smokers. J Periodontal Res 52:342-352
Ebersole, J L; Kryscio, R J; Campbell, C et al. (2017) Salivary and serum adiponectin and C-reactive protein levels in acute myocardial infarction related to body mass index and oral health. J Periodontal Res 52:419-427

Showing the most recent 10 out of 83 publications