Abstract

The Louisiana State University COBRE Core laboratory (LSU-MBCL) will be operationally housed within the existing centralized facilities of the Division of Biotechnology and Molecular Medicine (BIOMMED) and the Department of Pathobiological Sciences at the LSU School of Veterinary Medicine. The LSU-MBCL will concentrate its support functions on molecular biology/recombinant DNA services, bioinformatics and molecular evaluation of immunological parameters of mice. Molecular biological services and reagents will include standard services such as DNA sequencing, recombinant plasmid and cDNA library construction. Advanced services will include processing and analysis of microarray experiments using advanced bioinformatics capabilities offered in conjunction with the Bioinformatics Unit of the LSU Pennington Biomedical Research Center (PBRC-BU). In addition, LSU-MBCL will offer real-time PCR analysis of mouse cytokines, Fluorescent Activated Sorting (FACS) analysis, and confocal microscopy in collaboration with the School of Veterinary Medicine Centralized Facilities dedicated to FACS and Light Microscopy.
The specific aims of the LSU-MBCL are: (1) To provide advanced molecular biological know-how, reagents and services including bioinformatics to COBRE investigators. (2) To facilitate the use of advanced molecular immunoassays for the evaluation of immune responses of mice, and the use of other research resources of the LSU School of Veterinary Medicine.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
1P20RR020159-01
Application #
6972195
Study Section
Special Emphasis Panel (ZRR1-RI-5 (01))
Project Start
2004-07-01
Project End
2005-06-30
Budget Start
2004-07-01
Budget End
2005-06-30
Support Year
1
Fiscal Year
2004
Total Cost
$216,825
Indirect Cost

  Institution

Name
Louisiana State University A&M Col Baton Rouge
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
075050765
City
Baton Rouge
State
LA
Country
United States
Zip Code
70803

  Publications

Crossland, Nicholas A; Alvarez, Xavier; Embers, Monica E (2018) Late Disseminated Lyme Disease: Associated Pathology and Spirochete Persistence Posttreatment in Rhesus Macaques. Am J Pathol 188:672-682
Cheemarla, Nagarjuna R; Baños-Lara, Ma Del Rocío; Naidu, Shan et al. (2017) Neutrophils regulate the lung inflammatory response via ?? T cell infiltration in an experimental mouse model of human metapneumovirus infection. J Leukoc Biol 101:1383-1392
Cai, S; Batra, S; Del Piero, F et al. (2016) NLRP12 modulates host defense through IL-17A-CXCL1 axis. Mucosal Immunol 9:503-14
Cai, S; Batra, S; Langohr, I et al. (2016) IFN-? induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense. Mucosal Immunol 9:718-29
Phillips, Bonnie L; Mehra, Smriti; Ahsan, Muhammad H et al. (2015) LAG3 expression in active Mycobacterium tuberculosis infections. Am J Pathol 185:820-33
Pahar, Bapi; Pan, Diganta; Lala, Wendy et al. (2015) Transforming growth factor-?1 regulated phosphorylated AKT and interferon gamma expressions are associated with epithelial cell survival in rhesus macaque colon explants. Clin Immunol 158:8-18
Gautam, Uma Shankar; Mehra, Smriti; Kaushal, Deepak (2015) In-Vivo Gene Signatures of Mycobacterium tuberculosis in C3HeB/FeJ Mice. PLoS One 10:e0135208
Mehra, Smriti; Foreman, Taylor W; Didier, Peter J et al. (2015) The DosR Regulon Modulates Adaptive Immunity and Is Essential for Mycobacterium tuberculosis Persistence. Am J Respir Crit Care Med 191:1185-96
Baños-Lara, Ma Del Rocío; Harvey, Lindsey; Mendoza, Alexander et al. (2015) Impact and regulation of lambda interferon response in human metapneumovirus infection. J Virol 89:730-42
Caskey, John R; Embers, Monica E (2015) Persister Development by Borrelia burgdorferi Populations In Vitro. Antimicrob Agents Chemother 59:6288-95

Showing the most recent 10 out of 101 publications