This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Periodontal disease afflicts nearly 30% of the adult population and is a main cause of tooth loss. The susceptibility of the gingival tissues to plaque-induced periodontal disease may be influenced by the aging process. We hypothesize that aging of periodontal tissues causes changes in the expression of proteins critical for inflammation and tissue regeneration. This results in greater disease susceptibility and lower healing potential in aged subjects. To test this hypothesis, biopsies of gingival tissue from young and aged subjects will be used to examine the expression of inflammatory cytokines and growth factors. The results are expected to serve as a basis for developing novel approaches for therapeutic intervention in periodontitis that may lead to improved periodontal therapies and tooth retention. To this end, the following is the progress for year 1. Objective: To prepare a protocol to study the effects of aging on periodontal disease; to set up the laboratory with the necessary equipment; to become educated as a scientific investigator. Methods: Worked with the mentoring team to prepare the proposal for the COBRE and set up the lab; attended COBRE program sessions. Results: The proposal was approved by the External Advisory Committee (EAC) with minor revisions. The grant was subsequently approved by the NIH an amended award notice was recently received. The protocol also obtained approval by the IRB. Lab space was renovated and the laboratory is being set up, with most equipment already in place. Two research associates (100% and 50% effort) were hired. The PI and the associates are optimizing the laboratory techniques necessary for the project. The PI participated in COBRE sponsored activities, such as the monthly journal club, the grant seeker sessions, the comprehensive clinical training course, and progress reports and presentations. Discussion: The PI with constant cooperation with the mentoring team and the EAC have already clarified methodology issues. Pilot studies are being set-up and the research team is preparing to recruit patients.
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