This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Core will provide all COBRE scientists with technical support, state-of-the-art equipment, and essential molecular biology reagents, so that experiments which require a molecular biology approach can be carried out in a cost-effective way. The Core will provide a central DNA-sequencing facility, real-time PCR services to quantify mRNA expression, high-throughput PCR and genotyping services, and a central bank of vectors, plasmids, and bacteria for sharing. The Core will provide a central location for essential equipment, including an ABI Prism 3100 Genetic Analyzer (requested in the current application), a real-time PCR machine (in place), a Biomek FX Assay Work Station (liquid dispenser, in place), and 5 thermocyclers (in place), and other commonly used molecular biology equipment, such as an imaging system with the capability for densitometry analysis, bacteria incubators, and ultracentrifuges. The Core w II be staffed with experienced molecular biologists, who not only have hands-on experience in using this equipment, but also a proven track record in managing a core facility.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
1P20RR021940-01
Application #
7382247
Study Section
Special Emphasis Panel (ZRR1-RI-8 (01))
Project Start
2006-06-01
Project End
2007-04-30
Budget Start
2006-06-01
Budget End
2007-04-30
Support Year
1
Fiscal Year
2006
Total Cost
$71,493
Indirect Cost
Name
University of Kansas
Department
Pharmacology
Type
Schools of Medicine
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160
Wang, Yifeng; Ding, Wen-Xing; Li, Tiangang (2018) Cholesterol and bile acid-mediated regulation of autophagy in fatty liver diseases and atherosclerosis. Biochim Biophys Acta Mol Cell Biol Lipids 1863:726-733
Zhao, Jie; Adams, Abby; Roberts, Ben et al. (2018) Protein arginine methyl transferase 1- and Jumonji C domain-containing protein 6-dependent arginine methylation regulate hepatocyte nuclear factor 4 alpha expression and hepatocyte proliferation in mice. Hepatology 67:1109-1126
Butler, Merlin G; Hossain, Waheeda A; Tessman, Robert et al. (2018) Preliminary observations of mitochondrial dysfunction in Prader-Willi syndrome. Am J Med Genet A 176:2587-2594
Wang, Yifeng; Li, Jibiao; Matye, David et al. (2018) Bile acids regulate cysteine catabolism and glutathione regeneration to modulate hepatic sensitivity to oxidative injury. JCI Insight 3:
Wang, Yifeng; Matye, David; Nguyen, Nga et al. (2018) HNF4? Regulates CSAD to Couple Hepatic Taurine Production to Bile Acid Synthesis in Mice. Gene Expr 18:187-196
Kumar, Dhruv; New, Jacob; Vishwakarma, Vikalp et al. (2018) Cancer-Associated Fibroblasts Drive Glycolysis in a Targetable Signaling Loop Implicated in Head and Neck Squamous Cell Carcinoma Progression. Cancer Res 78:3769-3782
Borude, Prachi; Bhushan, Bharat; Apte, Udayan (2018) DNA Damage Response Regulates Initiation of Liver Regeneration Following Acetaminophen Overdose. Gene Expr 18:115-123
Huck, Ian; Beggs, Kevin; Apte, Udayan (2018) Paradoxical Protective Effect of Perfluorooctanesulfonic Acid Against High-Fat Diet-Induced Hepatic Steatosis in Mice. Int J Toxicol 37:383-392
Jiang, Lu; Fang, Pingping; Septer, Seth et al. (2018) Inhibition of Mast Cell Degranulation With Cromolyn Sodium Exhibits Organ-Specific Effects in Polycystic Kidney (PCK) Rats. Int J Toxicol 37:308-326
McCracken, Jennifer M; Chalise, Prabhakar; Briley, Shawn M et al. (2017) C57BL/6 Substrains Exhibit Different Responses to Acute Carbon Tetrachloride Exposure: Implications for Work Involving Transgenic Mice. Gene Expr 17:187-205

Showing the most recent 10 out of 366 publications