This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The Null-Mouse Core will maintain standing colonies of nuclear-receptor null-mice to serve the scientific needs of the five individual research projects, as well as those of the mentors and other investigators at the institution. Because numerous investigators will require nuclear-receptor null-mice, it is much more efficient and costeffective to have a 'bank' of null-mice. The intent is to have 5 breeding pairs of each null strain in the nullmouse core. When an investigator needs to use a specific null mouse, three pairs of breeders will be transferred to the investigator, and the investigator will start paying the mouse maintenance. This will also save time because, MTAs (material transfer agreements) won't need to be obtained, nor income animals quarantine time. At the present time, we have the following null colonies: PXR, AhR, RXRa, CAR, PPARa, Nr'2, HNF1a, FXR (whole-body and hepatocyte-specific) and hepatocyte-specific PPARy and HNF4a. For the projects of this proposal, SHP-null mice will be obtained from David Moore at Baylor (Dr Wang's mentor). In addition, Core C will have the technical capability to engineer other new knockouts or double knockouts as needed. Another feature of this Core will be to conduct routine genotyping experiments necessary to demonstrate the ongoing integrity of the null-mouse model, as well as to engineer double-null mice, and perform the genotyping. The other major role of this core is to isolate liver, prepare hepatocytes, and perform culture experiments upon request for the investigators of this COBRE.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Exploratory Grants (P20)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-RI-8 (01))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Kansas
Schools of Medicine
Kansas City
United States
Zip Code
Zhao, Jie; Adams, Abby; Roberts, Ben et al. (2018) Protein arginine methyl transferase 1- and Jumonji C domain-containing protein 6-dependent arginine methylation regulate hepatocyte nuclear factor 4 alpha expression and hepatocyte proliferation in mice. Hepatology 67:1109-1126
Butler, Merlin G; Hossain, Waheeda A; Tessman, Robert et al. (2018) Preliminary observations of mitochondrial dysfunction in Prader-Willi syndrome. Am J Med Genet A 176:2587-2594
Wang, Yifeng; Li, Jibiao; Matye, David et al. (2018) Bile acids regulate cysteine catabolism and glutathione regeneration to modulate hepatic sensitivity to oxidative injury. JCI Insight 3:
Wang, Yifeng; Matye, David; Nguyen, Nga et al. (2018) HNF4? Regulates CSAD to Couple Hepatic Taurine Production to Bile Acid Synthesis in Mice. Gene Expr 18:187-196
Kumar, Dhruv; New, Jacob; Vishwakarma, Vikalp et al. (2018) Cancer-Associated Fibroblasts Drive Glycolysis in a Targetable Signaling Loop Implicated in Head and Neck Squamous Cell Carcinoma Progression. Cancer Res 78:3769-3782
Borude, Prachi; Bhushan, Bharat; Apte, Udayan (2018) DNA Damage Response Regulates Initiation of Liver Regeneration Following Acetaminophen Overdose. Gene Expr 18:115-123
Huck, Ian; Beggs, Kevin; Apte, Udayan (2018) Paradoxical Protective Effect of Perfluorooctanesulfonic Acid Against High-Fat Diet-Induced Hepatic Steatosis in Mice. Int J Toxicol 37:383-392
Jiang, Lu; Fang, Pingping; Septer, Seth et al. (2018) Inhibition of Mast Cell Degranulation With Cromolyn Sodium Exhibits Organ-Specific Effects in Polycystic Kidney (PCK) Rats. Int J Toxicol 37:308-326
Wang, Yifeng; Ding, Wen-Xing; Li, Tiangang (2018) Cholesterol and bile acid-mediated regulation of autophagy in fatty liver diseases and atherosclerosis. Biochim Biophys Acta Mol Cell Biol Lipids 1863:726-733
Li, Jibiao; Chen, Cheng; Li, Yuan et al. (2017) Inhibition of insulin/PI3K/AKT signaling decreases adipose Sortilin 1 in mice and 3T3-L1 adipocytes. Biochim Biophys Acta Mol Basis Dis 1863:2924-2933

Showing the most recent 10 out of 366 publications