This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the Pennington Biomedical Research Centers Genomlcs Core Facility (PBRC-GCF) ).s to provide high-throughput DNA sequencing, real time PCR and microarray capabilities for the LSI) community and surrounding institutions in the State of Louisiana. The PBRC-GCF website (http://gcf.pbrc.edii/). made public during the spring of 2001, has been used extensively for automated sample submission, data retrieval and billing by several institutions in the region. The website is continually being modified 1:0 suit the needs of research staff at Pennington and the surrounding scientific community. We are equipped with one AB1 3700 (96-capillary) and two ABI 3100s (16-capillary) genetic analyzer(s) and have the capability to sequence more than Vz million basepairs a day. The PBRC-GCF micro array facility produces high-density micro arrays from mouse (Operon, 13,442 and recently updated 16,463 oligonucleotide libraries) and human (Compugen. 18,861 oligonucleotide library) and several cDNA libraries generated by scientists at PBRC. Slides are printed with a GeneMachine OmniGrid Microarrayer equipped with server arm and scanned using a GS1 Lumonics ScanArray 5000. Bioinformatics support will also be available to investigators and we are presently developing databases for microarray data and information following the guidelines outlined by MIAMK, an international effort to standardize microarray data to provide minimum information about a microarray experiment. The PBRC-GCF is equipped with robotics (Biomek FX robotic workstation, Beckman-Coulter) to support the high-throughput DNA sequencing and microarray technologies, and, two real-time PCR sequence detection systems (ABI 7700; ABI 7900) for verification of microarray results and quantitative RT-PCR. The PBRC-GCF is directed by Dr. Leslie P. Kozak and Dr. Robert A. KoZa arid managed by Susan Newman with assistance from Rebecca McCabe. In addition to services, the Core will develop new methodologies that will enhance the research activities of PBRC scientists. The initial development will be a CpG island microarray for the benefit of researchers working on cpigenetic changes in relation to adult disease.
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