This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The overall theme guiding this and future work is to better understand the interactions between age, physical activity, and obesity on mechanisms that regulate insulin action. Insulin resistance, diabetes, obesity, and cardiometabolic syndrome are closely related health problems that are present in ever-growing numbers of adults and children. It is well established that improvement of these conditions can be achieved throughifestyle modification, but the impact of specific programs may vary with the approach used and the target population. In this proposal we will determine whether the response of insulin action to aerobic exercise training varies with age and body fatness. Our goal is to compare exercise responses in children and adults. Thus, a scientifically valuable, yet practical outcome measure of insulin action is needed, particularly for children.
In Specific Aim 1 we will establish the validity, reproducibility, and sensitivity of a minimally invasive method for assessing meal glucose tolerance in children and young adults. A mixed meal containing carbohydrate, protein, and fat will be tested, making it more physiologically relevant than the standard oral glucose tolerance test, but less invasive than either the insulin clamp or intravenous glucose tolerance test (IVGTT). The results will be tested against the IVGTT in normal-weight and overweight/obese subjects. The validity and reproducibility of insulin sensitivity determined from the meal test in children has not been reported so this study is needed to evaluate this test for future studies.
In Specific Aim 2 we will compare the effects of a 16-week program of aerobic exercise training, and short-term detraining, on insulin sensitivity among normal weight and overweight children, and normal weight and obese young and older adults.
This aim builds upon recent findings that the response of insulin sensitivity to exercise may decline with age. Normal and overweight/obese subjects will remain weight-stable throughout the training program and will undergo testing at 1, 5 and 14-days after the training program. This will determine the acute effect of trainingon insulin action, the durability of the training effect, and the potential interactions of age and body fatness on insulin action. The outcomes of the proposed studies will increase our ability to obtain practical and meaningful measures of insulin action in children and adults, and will help determine whether specificexercise recommendations are needed for people based on age and body fatness.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
1P20RR024215-01
Application #
7610814
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Project Start
2007-09-15
Project End
2008-06-30
Budget Start
2007-09-15
Budget End
2008-06-30
Support Year
1
Fiscal Year
2007
Total Cost
$261,225
Indirect Cost
Name
University of Oklahoma Health Sciences Center
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
878648294
City
Oklahoma City
State
OK
Country
United States
Zip Code
73117
Short, Kevin R; Pratt, Lauren V; Teague, April M (2018) A single exercise session increases insulin sensitivity in normal weight and overweight/obese adolescents. Pediatr Diabetes :
Fu, Dongxu; Yu, Jeremy Y; Connell, Anna R et al. (2016) Beneficial Effects of Berberine on Oxidized LDL-Induced Cytotoxicity to Human Retinal Müller Cells. Invest Ophthalmol Vis Sci 57:3369-79
Fu, Dongxu; Yu, Jeremy Y; Yang, Shihe et al. (2016) Survival or death: a dual role for autophagy in stress-induced pericyte loss in diabetic retinopathy. Diabetologia 59:2251-61
Powell, Rebecca; Bubenshchikova, Ekaterina; Fukuyo, Yayoi et al. (2016) Wtip is required for proepicardial organ specification and cardiac left/right asymmetry in zebrafish. Mol Med Rep 14:2665-78
Basu, Arpita; Morris, Stacy; Nguyen, Angel et al. (2016) Effects of Dietary Strawberry Supplementation on Antioxidant Biomarkers in Obese Adults with Above Optimal Serum Lipids. J Nutr Metab 2016:3910630
Wang, Zhongxiao; Cheng, Rui; Lee, Kyungwon et al. (2015) Nanoparticle-mediated expression of a Wnt pathway inhibitor ameliorates ocular neovascularization. Arterioscler Thromb Vasc Biol 35:855-64
Tryggestad, Jeanie B; Wang, Joshua J; Zhang, Sarah X et al. (2015) Elevated plasma pigment epithelium-derived factor in children with type 2 diabetes mellitus is attributable to obesity. Pediatr Diabetes 16:600-5
Webb, Carol F; Ratliff, Michelle L; Powell, Rebecca et al. (2015) A developmentally plastic adult mouse kidney cell line spontaneously generates multiple adult kidney structures. Biochem Biophys Res Commun 463:1334-1340
Du, Mei; Otalora, Laura; Martin, Ashley A et al. (2015) Transgenic Mice Overexpressing Serum Retinol-Binding Protein Develop Progressive Retinal Degeneration through a Retinoid-Independent Mechanism. Mol Cell Biol 35:2771-89
Basu, Arpita; Yu, Jeremy Y; Jenkins, Alicia J et al. (2015) Trace elements as predictors of preeclampsia in type 1 diabetic pregnancy. Nutr Res 35:421-30

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