In most studies of aging of rodents, investigators have utilized male animals. Recently, it has been recognized that there are sex differences in age-associated changes of some physiological parameters. However, the breadth and, more importantly, the mechanisms of i these sex-based differences have not been explored. The purpose of this proposed Animal Core Resource is to provide female mice and rats of various ages to investigators currently involved in aging research, to determine whether or not there is a sex-associated difference in the age-associated parameter that they are investigating. The Animal Core Resource will not only provide female animals to expand their research, but will also characterize all female animals, either purchased by the Core or by the investigator, as to the status of the estrus cycle and sex hormone profile. This assessment will provide definitive information on the role of sex hormones in sex-based differences observed during aging. Once areas have been identified that clearly demonstrate sex differences in age-associated changes, the Core will provide hormonally deprived animals to further explore the role of endocrine hormones in this sex-based effect. In addition to providing female mice to investigators currently funded for aging research, both female and male rodents will be made available to-Junior Investigators (who may also be funded through the Research Development Core) as well as to senior investigators who are interested in expanding their funded research to include gerontology but do not have the funds to obtain preliminary data in aged animals. Finally, the Animal Core Resource will facilitate both maximal utilization of fresh tissues and maximal deposition of unused tissues in close cooperation with the Cell and Tissue Core by coordinating experiments among the various investigators who will be using the Animal Core Resource. The activities of the Animal Core Resource will not only allow expansion of ongoing aging research, but also will provide the impetus for new research in the basic scientific aspects of aging.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Center Core Grants (P30)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Allegheny University of Health Sciences
United States
Zip Code
Ray, Donna; Wu, Ailing; Wilkinson, J Erby et al. (2006) Aging in heterozygous Dnmt1-deficient mice: effects on survival, the DNA methylation genes, and the development of amyloidosis. J Gerontol A Biol Sci Med Sci 61:115-24
Mo, RuRan; Chen, Jun; Grolleau-Julius, Annabelle et al. (2005) Estrogen regulates CCR gene expression and function in T lymphocytes. J Immunol 174:6023-9
Mo, Ruran; Chen, Jun; Han, Yin et al. (2003) T cell chemokine receptor expression in aging. J Immunol 170:895-904
Chen, Jun; Mo, Ruran; Lescure, Pascal A et al. (2003) Aging is associated with increased T-cell chemokine expression in C57BL/6 mice. J Gerontol A Biol Sci Med Sci 58:975-83
Lorenzini, Antonello; Tresini, Maria; Mawal-Dewan, Madhu et al. (2002) Role of the Raf/MEK/ERK and the PI3K/Akt(PKB) pathways in fibroblast senescence. Exp Gerontol 37:1149-56
Yung, R; Ray, D; Eisenbraun, J K et al. (2001) Unexpected effects of a heterozygous dnmt1 null mutation on age-dependent DNA hypomethylation and autoimmunity. J Gerontol A Biol Sci Med Sci 56:B268-76
Tresini, M; Lorenzini, A; Frisoni, L et al. (2001) Lack of Elk-1 phosphorylation and dysregulation of the extracellular regulated kinase signaling pathway in senescent human fibroblast. Exp Cell Res 269:287-300
Lorenzini, A; Hrelia, S; Bordoni, A et al. (2001) Is increased arachidonic acid release a cause or a consequence of replicative senescence? Exp Gerontol 36:65-78
Severino, J; Allen, R G; Balin, S et al. (2000) Is beta-galactosidase staining a marker of senescence in vitro and in vivo? Exp Cell Res 257:162-71
Li, M; Walter, R; Torres, C et al. (2000) Impaired signal transduction in mitogen activated rat splenic lymphocytes during aging. Mech Ageing Dev 113:85-99

Showing the most recent 10 out of 13 publications