Transgenic animals give investigators a powerful system for either targeting the expression of a gene to a specific tissue(s) or obliterating the expression of a specific gene. Therefore, transgenic animals are an excellent tool for directly evaluating the role of a specific gene (or genes) in an age-related disease or in aging. The Transgenic Core will provide all services and services and resources required to produce transgenic mice, transgenic rats, and transgenic mice with gene knockouts for Center investigators. Because the techniques for producing transgenic rats or mice with gene knockouts are not currently available at UTHSC-SA, an important goal of this Core is to develop these techniques.
The specific aims of the Core are as follow: 1. To produce transgenic mice by microinjection and to identify the transgenic mice by PCR and DNA hybridization techniques. The number and integration sites of the transgene also will be determined by examining DNA from the transgenic mice. 2. To develop the techniques for producing transgenic rats by microinjection and to use these techniques to produce transgenic rats for Center investigators. Transgenic rats will be identified by PCR and DNA hybridization techniques, and the number and integration sites of the transgene will be determined. 3. To develop the techniques for producing transgenic mice with gene knockouts using homologous recombination and to use these techniques to produce transgenic knockout mice for Center investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Center Core Grants (P30)
Project #
5P30AG013319-05
Application #
6201044
Study Section
Project Start
1999-08-01
Project End
2000-06-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of Texas Health Science Center San Antonio
Department
Type
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229
Weiss, Roxanne; Fernandez, Elizabeth; Liu, Yuhong et al. (2018) Metformin reduces glucose intolerance caused by rapamycin treatment in genetically heterogeneous female mice. Aging (Albany NY) :
Qin, Kunhua; Zhang, Ning; Zhang, Zhao et al. (2018) SIRT6-mediated transcriptional suppression of Txnip is critical for pancreatic beta cell function and survival in mice. Diabetologia 61:906-918
Salmon, Adam B; Dorigatti, Jonathan; Huber, Hillary F et al. (2018) Maternal nutrient restriction in baboon programs later-life cellular growth and respiration of cultured skin fibroblasts: a potential model for the study of aging-programming interactions. Geroscience 40:269-278
Gelfond, Jonathan; Goros, Martin; Hernandez, Brian et al. (2018) A System for an Accountable Data Analysis Process in R. R J 10:6-21
Sills, Aubrey M; Artavia, Joselyn M; DeRosa, Brian D et al. (2018) Long-term treatment with the mTOR inhibitor rapamycin has minor effect on clinical laboratory markers in middle-aged marmosets. Am J Primatol :e22927
Xu, Ming; Pirtskhalava, Tamar; Farr, Joshua N et al. (2018) Senolytics improve physical function and increase lifespan in old age. Nat Med 24:1246-1256
Unnikrishnan, Archana; Hadad, Niran; Masser, Dustin R et al. (2018) Revisiting the genomic hypomethylation hypothesis of aging. Ann N Y Acad Sci 1418:69-79
Van Skike, Candice E; Jahrling, Jordan B; Olson, Angela B et al. (2018) Inhibition of mTOR protects the blood-brain barrier in models of Alzheimer's disease and vascular cognitive impairment. Am J Physiol Heart Circ Physiol 314:H693-H703
Mao, Kai; Quipildor, Gabriela Farias; Tabrizian, Tahmineh et al. (2018) Late-life targeting of the IGF-1 receptor improves healthspan and lifespan in female mice. Nat Commun 9:2394
Lee, Hak Joo; Feliers, Denis; Barnes, Jeffrey L et al. (2018) Hydrogen sulfide ameliorates aging-associated changes in the kidney. Geroscience 40:163-176

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