The Older American Independence Center (OAIC) Metabolism and Biomarkers Core in collaboration with all other Cores, utilizes translational research to determine specific mechanisms of sarcopenia and the cause of reduced physical function in elderly populations. Sarcopenia is characterized by a progressive deterioration in various physiological and metabolic processes and is associated with lower physical function. Analyses performed by the Core focus on mitochondrial function, inflammation, oxidative stress, apoptosis and autophagy, biological factors implicated to cause aging. The Core supports the hypothesis that mitochondrial dysfunction, inflammation, oxidative stress and deregulation of apoptosis and autophagy are major causes of sarcopenia and disability. Supported research proposals will contain refined questions and utilize selected methodologies addressing potential causes of sarcopenia and altered physical function. Importantly, the Core is a central facility for acquiring research data and new laboratory skills. Training and instruction is provided either one on one or through organized workshops. By acquiring new laboratory skills and techniques Junior investigators and Pepper Scholars can further develop their research interests independently. In addition, the diversity of research experience and skills among personnel within the core as well as scientists utilizing its facilities provides a rich environment for scientific discussion and collaborations. The Core also provides consultations to scientists who are either interested in new areas of research or unfamiliar with certain techniques. Thus, this Core provides the infrastructure and training necessary to develop our understanding of the mechanisms contributing to the aging process. Furthermore, we are committed to fostering novel technologies in our pursuit of deciphering the central role that mitochondrial dysfunction plays in the pathogenesis of diseases and aging. To this end we have recently developed innovative intravital-multiphoton excitation laser-scanning microscopy and high-resolution respirometry techniques to assess mitochondrial function in intact freshly isolated small (20-40 mg) muscle samples. Thus, we are now able to determine mitochondrial function in-situ, which is more reflective of the natural state. Other areas of focus include inflammation, oxidative stress (including iron deposition), apoptosis and autophagy biomarkers. In summary, by measuring a small selected set of cellular and molecular markers in skeletal muscle tissue we can assess a unique and comprehensive spectrum of age-related alterations with the goal of determining the mechanisms contributing to sarcopenia.

Public Health Relevance

The Core assays specific biological functions and pathways believed to be causal to sarcopenia and aging, instigates interventions designed to improve muscle mass and function, and determines whether these potential risk factors for disability are modifiable. Hence, the Core is a central facility for obtaining research data, providing workshops, and training in laboratory procedures for numerous Junior investigators and Pepper Scholars. This Core provides the infrastructure, highly qualified personnel, training, consultative and collaborative scientific expertise and a specific spectrum of established methodologies of biochemistry and molecular biology.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Center Core Grants (P30)
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Special Emphasis Panel (ZAG1-ZIJ-8 (M1))
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University of Florida
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Shenkman, Elizabeth; Hurt, Myra; Hogan, William et al. (2018) OneFlorida Clinical Research Consortium: Linking a Clinical and Translational Science Institute With a Community-Based Distributive Medical Education Model. Acad Med 93:451-455
Hernandez, Abbi R; Hernandez, Caesar M; Campos, Keila et al. (2018) A Ketogenic Diet Improves Cognition and Has Biochemical Effects in Prefrontal Cortex That Are Dissociable From Hippocampus. Front Aging Neurosci 10:391
Brakenridge, Scott C; Efron, Philip A; Stortz, Julie A et al. (2018) The impact of age on the innate immune response and outcomes after severe sepsis/septic shock in trauma and surgical intensive care unit patients. J Trauma Acute Care Surg 85:247-255
Dahodwala, Nabila; Shah, Krunal; He, Ying et al. (2018) Sex disparities in access to caregiving in Parkinson disease. Neurology 90:e48-e54
Buford, Thomas W; Manini, Todd M; Kairalla, John A et al. (2018) Mitochondrial DNA Sequence Variants Associated With Blood Pressure Among 2 Cohorts of Older Adults. J Am Heart Assoc 7:e010009
Liu, Xinyue; Carney, Paul R; Bussing, Regina et al. (2018) Stimulants Do Not Increase the Risk of Seizure-Related Hospitalizations in Children with Epilepsy. J Child Adolesc Psychopharmacol 28:111-116
Chen, Haiying; Rejeski, W Jack; Gill, Thomas M et al. (2018) A Comparison of Self-report Indices of Major Mobility Disability to Failure on the 400-m Walk Test: The LIFE Study. J Gerontol A Biol Sci Med Sci 73:513-518
Bihorac, Azra; Ozrazgat-Baslanti, Tezcan; Ebadi, Ashkan et al. (2018) MySurgeryRisk: Development and Validation of a Machine-learning Risk Algorithm for Major Complications and Death After Surgery. Ann Surg :
Rillamas-Sun, Eileen; LaMonte, Michael J; Evenson, Kelly R et al. (2018) The Influence of Physical Activity and Sedentary Behavior on Living to Age 85 Years Without Disease and Disability in Older Women. J Gerontol A Biol Sci Med Sci 73:1525-1531
Moore, Brenda D; Martin, Jason; de Mena, Lorena et al. (2018) Short A? peptides attenuate A?42 toxicity in vivo. J Exp Med 215:283-301

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