Core - Molecular Virology

Luciw, Paul

Abstract

The MOLECULAR VIROLOGY CORE in this CFAR proposal will provide equipment, materials, and expertise for supporting research projects that require molecular cloning, radioactive and non- radioactive probes, synthetic oligonucleotides, expression vector systems, DNA sequencing, and analysis of viral and cellular macromolecules (polypeptides, RNA, and DNA). These projects involve several retroviruses including human immunodeficiency viruses types 1 and 2 (HIV-1 and HIV-2), simian immunodeficiency viruses (SIV) from rhesus macaque and sooty mangabey monkeys, feline T-lymphotropic virus (FTLV), simian AIDS-associated retrovirus (SRV, a type D retrovirus), bovine immunodeficiency-like virus (BIV), visna virus, and caprine-arthritis-encephalitis virus (CAEV) as well as several DNA viruses (e.g., rhesus and human cytomegaloviruses, rhesus Epstein-Barr virus, rhesus denovirus, vaccina virus). These activities in the MOLECULAR VIROLOGY CORE involve many direct interactions with investigators in both the PRIMATE CENTER and the CLINICAL VIROLOGY CORES.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Center Core Grants (P30)
• Project #: 5P30AI027732-05
• Application #: 3791379
• Support Year: 5
• Fiscal Year: 1992

Institution

• Name: University of California Davis
• DUNS #: 094878337
• City: Davis
• State: CA
• Country: United States
• Zip Code: 95618

Publications

Yamanaka, M K; Yilma, T (1998) Altered plaque formation by recombinant vaccinia virus expressing simian immunodeficiency virus Nef. J Virol 72:5291-5

Giavedoni, L D; Yilma, T (1996) Construction and characterization of replication-competent simian immunodeficiency virus vectors that express gamma interferon. J Virol 70:2247-51

Gardner, M; Rosenthal, A; Jennings, M et al. (1995) Passive immunization of rhesus macaques against SIV infection and disease. AIDS Res Hum Retroviruses 11:843-54

Luciw, P A; Pratt-Lowe, E; Shaw, K E et al. (1995) Persistent infection of rhesus macaques with T-cell-line-tropic and macrophage-tropic clones of simian/human immunodeficiency viruses (SHIV). Proc Natl Acad Sci U S A 92:7490-4

Gardner, M B; Rosenthal, A; Jennings, M et al. (1994) Passive immunization of macaques against SIV infection. J Med Primatol 23:164-74

Ahmad, S; Lohman, B; Marthas, M et al. (1994) Reduced virus load in rhesus macaques immunized with recombinant gp160 and challenged with simian immunodeficiency virus. AIDS Res Hum Retroviruses 10:195-204

Herchenroder, O; Renne, R; Loncar, D et al. (1994) Isolation, cloning, and sequencing of simian foamy viruses from chimpanzees (SFVcpz): high homology to human foamy virus (HFV). Virology 201:187-99

Anderson, D E; Malley, A; Benjamini, E et al. (1994) Hypervariable epitope constructs as a means of accounting for epitope variability. Vaccine 12:736-40

Torres, J V; Malley, A; Banapour, B et al. (1993) An epitope on the surface envelope glycoprotein (gp130) of simian immunodeficiency virus (SIVmac) involved in viral neutralization and T cell activation. AIDS Res Hum Retroviruses 9:423-30

Yamamoto, J K; Hohdatsu, T; Olmsted, R A et al. (1993) Experimental vaccine protection against homologous and heterologous strains of feline immunodeficiency virus. J Virol 67:601-5