Core - Virology

Abstract

The purpose of the Virology Core, located at the Aaron Diamond AIDS Research Center, is to provide HIV virology support to researchers in the NYU CFAR. Several virologic tests are offered routinely, including HIV isolation, p24 antigen assay, diagnostic PCR, HIV quantitation by culture and PCR, determination of plasma viremia by the branched-chain DNA amplification method, and phenotyping of patient viruses. Additional HIV tests are performed by special arrangement, including HIV propagation and titration, drug sensitivity testing, antibody neutralization of laboratory strains or field isolates, reverse transcriptase assays, and PCR-based nucleotide sequencing. Heteroduplex mobility shift assay, ELISA's to measure CD4-gp120 binding, epitope mapping of antibodies, and several in situ techniques are also in the repertoire of the Virology Core. These techniques and our overall expertise on HIV virology will be utilized to enhance the research activities in the NYU CFAR, particularly in the areas of pathogenesis, transmission, and vaccine research.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Center Core Grants (P30)
• Project #: 3P30AI027742-09S1
• Application #: 2755620
• Budget Start: 1997-10-01
• Budget End: 1998-09-30
• Support Year: 9
• Fiscal Year: 1998

Institution

• Name: New York University
• City: New York
• State: NY
• Country: United States
• Zip Code: 10016

Publications

Gornalusse, German G; Mummidi, Srinivas; Gaitan, Alvaro A et al. (2015) Epigenetic mechanisms, T-cell activation, and CCR5 genetics interact to regulate T-cell expression of CCR5, the major HIV-1 coreceptor. Proc Natl Acad Sci U S A 112:E4762-71

Miller, Elizabeth A; Gopal, Ramya; Valdes, Vanessa et al. (2015) Soluble CD40 ligand contributes to dendritic cell-mediated T-cell dysfunction in HIV-1 infection. AIDS 29:1287-96

Weiden, Michael D; Hoshino, Satomi; Levy, David N et al. (2014) Adenosine deaminase acting on RNA-1 (ADAR1) inhibits HIV-1 replication in human alveolar macrophages. PLoS One 9:e108476

Ryndak, Michelle B; Singh, Krishna K; Peng, Zhengyu et al. (2014) Transcriptional profiling of Mycobacterium tuberculosis replicating ex vivo in blood from HIV- and HIV+ subjects. PLoS One 9:e94939

Phelan, Joan A; Abrams, William R; Norman, Robert G et al. (2014) Design aspects of a case-control clinical investigation of the effect of HIV on oral and gastrointestinal soluble innate factors and microbes. PLoS One 9:e112901

Sivapalasingam, Sumathi; Mendillo, Megan; Ahmed, Aabid et al. (2014) The importance of caregivers in the outcome of pediatric HIV management, Mombasa, Kenya. AIDS Care 26:425-33

Weiser, Keren; Barton, Meredith; Gershoony, Dafna et al. (2013) HIV's Nef interacts with ?-catenin of the Wnt signaling pathway in HEK293 cells. PLoS One 8:e77865

O'Brien, Meagan; Montenont, Emilie; Hu, Liang et al. (2013) Aspirin attenuates platelet activation and immune activation in HIV-1-infected subjects on antiretroviral therapy: a pilot study. J Acquir Immune Defic Syndr 63:280-8

Rich, Josiah D; DiClemente, Ralph; Levy, Judith et al. (2013) Correctional facilities as partners in reducing HIV disparities. J Acquir Immune Defic Syndr 63 Suppl 1:S49-53

Chiu, Yung-Nung; Sampson, Jared M; Jiang, Xunqing et al. (2012) Skin tattooing as a novel approach for DNA vaccine delivery. J Vis Exp :