Abstract

This proposal is for the establishment of a Flow Cytometry Core Facility (FCCF) within the proposed Center for AIDS Research at the University of Alabama at Birmingham. The establishment of the CFAR FCCF will provide access to all investigators performing AIDS- related research using potentially infectious material to a state of the art, computer controlled four parameter (2 channels of fluorescence plus forward and orthagonal light scatter) flow cytometer/sorter. This unit will be equipped with a Coherent Innova 2 watt argon-ion laser, which when operating at 488 nm will allow the discrimination of lymphocytes, monocytes, granulocytes, erythrocytes and dead cells using bivariate light scattering analysis. In addition, this laser line will excite both fluorescein isothiocyanate (FITC) and phycoerythrin (PE), and one or two color immunofluorescence analyses will be possible. Cell cycle analyses can also be performed using this cytometer using 488 nm light. Acridine orange (AO) staining of permeabilized cells will be used to independently quantitate the DNA and RNA present in individual cells. Propidium iodide (PI) staining is an additional method for quantitating cellular DNA, and offers the advantage that it can be combined with FITC immunofluorescence to yield data on cycle-dependent antigen expression. These types of experiments are not currently possible on HIV-infected material at UAB.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI027767-05
Application #
3791410
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Type
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294

  Publications

Ladowski, Joseph M; Reyes, Luz M; Martens, Gregory R et al. (2018) Swine Leukocyte Antigen Class II Is a Xenoantigen. Transplantation 102:249-254
Owens, Michael A; Parker, Romy; Rainey, Rachael L et al. (2018) Enhanced facilitation and diminished inhibition characterizes the pronociceptive endogenous pain modulatory balance of persons living with HIV and chronic pain. J Neurovirol :
Chakraborty, Asmi; Dorsett, Kaitlyn A; Trummell, Hoa Q et al. (2018) ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage. J Biol Chem 293:984-994
Stringer, Kristi Lynn; Azuero, Andres; Ott, Corilyn et al. (2018) Feasibility and Acceptability of Real-Time Antiretroviral Adherence Monitoring among Depressed Women Living with HIV in the Deep South of the US. AIDS Behav :
Merlin, Jessica S; Long, Dustin; Becker, William C et al. (2018) Brief Report: The Association of Chronic Pain and Long-Term Opioid Therapy With HIV Treatment Outcomes. J Acquir Immune Defic Syndr 79:77-82
Hamilton, Jennie A; Wu, Qi; Yang, PingAr et al. (2018) Cutting Edge: Intracellular IFN-? and Distinct Type I IFN Expression Patterns in Circulating Systemic Lupus Erythematosus B Cells. J Immunol 201:2203-2208
Stafman, Laura L; Mruthyunjayappa, Smitha; Waters, Alicia M et al. (2018) Targeting PIM kinase as a therapeutic strategy in human hepatoblastoma. Oncotarget 9:22665-22679
Yang, Zhenhua; Shah, Kushani; Busby, Theodore et al. (2018) Hijacking a key chromatin modulator creates epigenetic vulnerability for MYC-driven cancer. J Clin Invest 128:3605-3618
Nag, Mukta; De Paris, Kristina; E Fogle, Jonathan (2018) Epigenetic Modulation of CD8? T Cell Function in Lentivirus Infections: A Review. Viruses 10:
Barr, Fiona D; Ochsenbauer, Christina; Wira, Charles R et al. (2018) Neutrophil extracellular traps prevent HIV infection in the female genital tract. Mucosal Immunol 11:1420-1428

Showing the most recent 10 out of 955 publications