The overall goal of the Gene and Cellular Therapy Core (CoreG) is to provide support for HIV/AIDS-related research requiring stem cells, gene delivery vectors and technical expertise for efficient genetic modification of stem cells. Recent advancements in gene delivery vector systems and stem cell technologies have enabled genetic modification of human hematopoietic stem/progenitor cells (HSPC) to resist HIV infection. The Gene and Cellular Therapy Core is established to meet the increasing demand to promote and facilitate basic and translational research in this area by providing UCLA CFAR investigators and their domestic and international collaborators with highly purified and well characterized human CD34+ HSPC, embryonic stem cells (hESC), induced pluripotent stem cells (iPSC), human fetal tissues and lentiviral vector technologies that enable efficient genetic engineering of stem cells to resist HIV infection. The Core also provides consultation for researchers with limited experience in stem cell and viral vector technologies, in particular early stage investigators. As the use of stem cells and vector technology requires specialized expertise and resources for efficient genetic engineering of different types of stem cells, offering access to these technologies can significantly facilitate and expand the scope of UCLA CFAR research activities. Our services are more cost-effective than utilizing the limited commercial sources. Further value is added by customized technical support available from accessible and knowledgeable core staffs who can work closely with investigators to troubleshoot and optimize experiments, assist with institutional regulatory compliance documents and who are actively engaged in development and application of stem cell and vector technologies. These core services will facilitate translation of stem cell and gene therapy-related HIV research into therapeutic applications.

Public Health Relevance

The UCLA CFAR Gene and Cellular Therapy Core provides support for human stem cell-base3d gene therapy research. The core services and technical expertise can facilitate research efforts to make rapid progress towards providing a new therapy for HIV infected individuals to stably control HIV infection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI028697-25
Application #
8631026
Study Section
Special Emphasis Panel (ZAI1-UKS-A)
Project Start
2014-03-01
Project End
2018-02-28
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
25
Fiscal Year
2014
Total Cost
$111,435
Indirect Cost
$22,360
Name
University of California Los Angeles
Department
Type
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Shannon, Chelsea L; Klausner, Jeffrey D (2018) The growing epidemic of sexually transmitted infections in adolescents: a neglected population. Curr Opin Pediatr 30:137-143
Sun, Jie; He, Xin; Zhu, Yinghui et al. (2018) SIRT1 Activation Disrupts Maintenance of Myelodysplastic Syndrome Stem and Progenitor Cells by Restoring TET2 Function. Cell Stem Cell 23:355-369.e9
Withers, Keenan; Bristow, Clare; Nguyen, Minh et al. (2018) A field evaluation of a rapid dual immunoassay for human immunodeficiency virus and syphilis antibodies, Hanoi, Vietnam. Int J STD AIDS :956462418802685
Bristow, Claire C; Shannon, Chelsea; Herbst de Cortina, Sasha et al. (2018) Use of Oral Fluid With a Rapid Treponemal Test for Syphilis Evaluation. Sex Transm Dis 45:e65-e67
Bristow, Claire C; Kojima, Noah; Lee, Sung-Jae et al. (2018) HIV and syphilis testing preferences among men who have sex with men and among transgender women in Lima, Peru. PLoS One 13:e0206204
Beymer, Matthew R; DeVost, Michelle A; Weiss, Robert E et al. (2018) Does HIV pre-exposure prophylaxis use lead to a higher incidence of sexually transmitted infections? A case-crossover study of men who have sex with men in Los Angeles, California. Sex Transm Infect 94:457-462
Tylee, Daniel S; Sun, Jiayin; Hess, Jonathan L et al. (2018) Genetic correlations among psychiatric and immune-related phenotypes based on genome-wide association data. Am J Med Genet B Neuropsychiatr Genet 177:641-657
Alban, Tyler J; Alvarado, Alvaro G; Sorensen, Mia D et al. (2018) Global immune fingerprinting in glioblastoma patient peripheral blood reveals immune-suppression signatures associated with prognosis. JCI Insight 3:
Degtyar, Aleksandra; George, Paul E; Mallma, Patricia et al. (2018) Sexual Risk, Behavior, and HIV Testing and Status among Male and Transgender Women Sex Workers and their Clients in Lima, Peru. Int J Sex Health 30:81-91
Dovel, Kathryn; Shaba, Frackson; Nyirenda, Mike et al. (2018) Evaluating the integration of HIV self-testing into low-resource health systems: study protocol for a cluster-randomized control trial from EQUIP Innovations. Trials 19:498

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