Core - Gene Therapy

Poeschla, Eric M.

Abstract

The gene therapy core will support growing interest in the use of lentivirus-based retroviral vectors. HIV-based lentiviral vectors and standard oncoretrovirus-derived vectors will be provided for experimental studies of lentiviral biology, including studies of non-dividing and post- mitotic target cells. Investigations into the potential of these vectors for therapeutic gene transfer will also be enabled. In addition, assistance with the design, regulatory review and implementation of human gene therapy trials will be provided. The Core will employ a full time technician who will assist with services that include maintenance and cryopreservation of cell lines, preparation and use of plasmids and transfection reagents, transfection, vector harvest, cryopreservation and titration, assays for replication-competent retrovirus, record keeping for vector stocks and cell lines, and facilitation of communication and materials transfer to investigators.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Center Core Grants (P30)
• Project #: 3P30AI036214-08S1
• Application #: 6500685
• Project Start: 2001-09-30
• Project End: 2002-05-31
• Support Year: 8
• Fiscal Year: 2001
• Total Cost: $180,480

Institution

• Name: University of California San Diego
• DUNS #: 077758407
• City: La Jolla
• State: CA
• Country: United States
• Zip Code: 92093

Publications

Naticchia, Matthew R; Laubach, Logan K; Tota, Ember M et al. (2018) Embryonic Stem Cell Engineering with a Glycomimetic FGF2/BMP4 Co-Receptor Drives Mesodermal Differentiation in a Three-Dimensional Culture. ACS Chem Biol 13:2880-2887

Blumenthal, Jill; Jain, Sonia; Mulvihill, Evan et al. (2018) Perceived versus calculated HIV risk: Implications for Pre-exposure prophylaxis uptake in a randomized trial of men who have sex with men. J Acquir Immune Defic Syndr :

Kardava, Lela; Sohn, Haewon; Youn, Christine et al. (2018) IgG3 regulates tissue-like memory B cells in HIV-infected individuals. Nat Immunol 19:1001-1012

Wagner, Karla D; Syvertsen, Jennifer L; Verdugo, Silvia R et al. (2018) A mixed methods study of the social support networks of female sex workers and their primary noncommercial male partners in Tijuana, Mexico. J Mix Methods Res 12:437-457

Bastos, Francisco I; Bastos, Leonardo Soares; Coutinho, Carolina et al. (2018) HIV, HCV, HBV, and syphilis among transgender women from Brazil: Assessing different methods to adjust infection rates of a hard-to-reach, sparse population. Medicine (Baltimore) 97:S16-S24

Cepeda, Javier A; Eritsyan, Ksenia; Vickerman, Peter et al. (2018) Potential impact of implementing and scaling up harm reduction and antiretroviral therapy on HIV prevalence and mortality and overdose deaths among people who inject drugs in two Russian cities: a modelling study. Lancet HIV 5:e578-e587

Namazi, Golnaz; Fajnzylber, Jesse M; Aga, Evgenia et al. (2018) The Control of HIV After Antiretroviral Medication Pause (CHAMP) Study: Posttreatment Controllers Identified From 14 Clinical Studies. J Infect Dis 218:1954-1963

Lada, Steven M; Huang, Karissa; VanBelzen, D Jake et al. (2018) Quantitation of Integrated HIV Provirus by Pulsed-Field Gel Electrophoresis and Droplet Digital PCR. J Clin Microbiol 56:

Huang, Mia L; Michalak, Austen L; Fisher, Christopher J et al. (2018) Small Molecule Antagonist of Cell Surface Glycosaminoglycans Restricts Mouse Embryonic Stem Cells in a Pluripotent State. Stem Cells 36:45-54

Skaathun, Britt; Voisin, Dexter R; Cornwell, Benjamin et al. (2018) A Longitudinal Examination of Factors Associated with Network Bridging Among YMSM: Implications for HIV Prevention. AIDS Behav :