The Molecular Biology Core is designed to expedite access to basic molecular biology techniques, resources, and training needed by investigators exploring problems in HIV replication, pathogenesis, therapy, and immunoprophylaxis. A particular emphasis, is the support of developmental projects and investigators just entering the field of HIV research. The Core has four specific objectives: (1) The Core supports research requiring RNA and DMA sequence information. Low-cost, rapid, accurate DNA sequencing is provided, along with special expertise in sequencing of HIV-1, HIV-2, SIV, and other lentiviruses. Training in preparation and sequencing for custom applications, and optimization of sequencing under specialized conditions for requested projects is supported. (2) The Core supports other aspects of molecular biology research. This includes amplification and hybridization techniques for detection and quantification (plasma, tissue and culture samples) for AIDS-related viruses and vectors including HIV-1, HIV-2, SIV, EBV, KSHV, and HCV viruses, as well as MuLV-, HIV-1-, HIV-2-, and FIV-based vectors. (3) The Core provides support for gene-silencing technology. This includes siRNA design, synthesis, and vector construction, as well as lentiviral vectors for siRNA/shRNA expression. (4) The Core supports other aspects of molecular biology needed for HIV research. This includes distribution of specialty reagents (primers, plasmids, maps, other) and assistance with design of projects involving PCR and/or cloning. The Core also supports training seminars, and provides one-on-one training for fellows and students in sequencing techniques, methods, and techniques related to miRNA and siRNA, and QC and kinetic real-time PCR. Mentoring is provided to developmental investigators. In addition, the Core assists in acquisition, support, and administration of computational infrastructure, and facilitates access to third-party molecular biology software needed by HIV/AIDS investigators. Past and ongoing molecular biology support of UCSD research efforts illustrates the Core's usefulness. Since the last competitive renewal in 2002, work performed by the Molecular Biology Core has been identified by 111 different investigators who have used the Core in generating 92 publications and 40 new or renewal grant applications. Research efforts supported by the Core have increased our understanding of viral resistance, viral variation and compartmentalization, the immunopathogenesis of disease, and new approaches for treatment and immunoprophylaxis. In summary, the Molecular Biology Core is a valuable, productive, and popular core resource for the UCSD CFAR.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Center Core Grants (P30)
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Special Emphasis Panel (ZAI1)
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University of California San Diego
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White, Cory H; Beliakova-Bethell, Nadejda; Lada, Steven M et al. (2018) Transcriptional Modulation of Human Endogenous Retroviruses in Primary CD4+ T Cells Following Vorinostat Treatment. Front Immunol 9:603
Jiang, Wei; Luo, Zhenwu; Martin, Lisa et al. (2018) Drug Use is Associated with Anti-CD4 IgG-mediated CD4+ T Cell Death and Poor CD4+ T Cell Recovery in Viral-suppressive HIV-infected Individuals Under Antiretroviral Therapy. Curr HIV Res 16:143-150
Gianella, Sara; Marconi, Vincent C; Berzins, Baiba et al. (2018) Genital HIV-1 Shedding With Dolutegravir (DTG) Plus Lamivudine (3TC) Dual Therapy. J Acquir Immune Defic Syndr 79:e112-e114
Innes, Steve; Patel, Kunjal (2018) Noncommunicable diseases in adolescents with perinatally acquired HIV-1 infection in high-income and low-income settings. Curr Opin HIV AIDS 13:187-195
Morales, Mario; Rafful, Claudia; Gaines, Tommi L et al. (2018) Factors associated with extrajudicial arrest for syringe possession: results of a department-wide survey of municipal police in Tijuana, Mexico. BMC Int Health Hum Rights 18:36
Rhodes, Tim (2018) The becoming of methadone in Kenya: How an intervention's implementation constitutes recovery potential. Soc Sci Med 201:71-79
Christensen-Quick, Aaron; Chaillon, Antoine; Yek, Christina et al. (2018) Influenza Vaccination Can Broadly Activate the HIV Reservoir During Antiretroviral Therapy. J Acquir Immune Defic Syndr 79:e104-e107
Ben Hamida, Amen; Rafful, Claudia; Jain, Sonia et al. (2018) Non-injection Drug Use and Injection Initiation Assistance among People Who Inject Drugs in Tijuana, Mexico. J Urban Health 95:83-90
Dillon, Stephanie M; Guo, Kejun; Austin, Gregory L et al. (2018) A compartmentalized type I interferon response in the gut during chronic HIV-1 infection is associated with immunopathogenesis. AIDS 32:1599-1611
Groessl, Erik J; Ganiats, Theodore G; Hillery, Naomi et al. (2018) Cost analysis of rapid diagnostics for drug-resistant tuberculosis. BMC Infect Dis 18:102

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