The Molecular Biology Core is designed to expedite access to basic molecular biology techniques, resources, and training needed by investigators exploring problems in HIV replication, pathogenesis, therapy, and immunoprophylaxis. A particular emphasis, is the support of developmental projects and investigators just entering the field of HIV research. The Core has four specific objectives: (1) The Core supports research requiring RNA and DMA sequence information. Low-cost, rapid, accurate DNA sequencing is provided, along with special expertise in sequencing of HIV-1, HIV-2, SIV, and other lentiviruses. Training in preparation and sequencing for custom applications, and optimization of sequencing under specialized conditions for requested projects is supported. (2) The Core supports other aspects of molecular biology research. This includes amplification and hybridization techniques for detection and quantification (plasma, tissue and culture samples) for AIDS-related viruses and vectors including HIV-1, HIV-2, SIV, EBV, KSHV, and HCV viruses, as well as MuLV-, HIV-1-, HIV-2-, and FIV-based vectors. (3) The Core provides support for gene-silencing technology. This includes siRNA design, synthesis, and vector construction, as well as lentiviral vectors for siRNA/shRNA expression. (4) The Core supports other aspects of molecular biology needed for HIV research. This includes distribution of specialty reagents (primers, plasmids, maps, other) and assistance with design of projects involving PCR and/or cloning. The Core also supports training seminars, and provides one-on-one training for fellows and students in sequencing techniques, methods, and techniques related to miRNA and siRNA, and QC and kinetic real-time PCR. Mentoring is provided to developmental investigators. In addition, the Core assists in acquisition, support, and administration of computational infrastructure, and facilitates access to third-party molecular biology software needed by HIV/AIDS investigators. Past and ongoing molecular biology support of UCSD research efforts illustrates the Core's usefulness. Since the last competitive renewal in 2002, work performed by the Molecular Biology Core has been identified by 111 different investigators who have used the Core in generating 92 publications and 40 new or renewal grant applications. Research efforts supported by the Core have increased our understanding of viral resistance, viral variation and compartmentalization, the immunopathogenesis of disease, and new approaches for treatment and immunoprophylaxis. In summary, the Molecular Biology Core is a valuable, productive, and popular core resource for the UCSD CFAR.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI036214-17
Application #
8080821
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2010-06-01
Budget End
2011-05-31
Support Year
17
Fiscal Year
2010
Total Cost
$291,651
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Chaillon, Antoine; Gianella, Sara; Lada, Steven M et al. (2018) Size, Composition, and Evolution of HIV DNA Populations during Early Antiretroviral Therapy and Intensification with Maraviroc. J Virol 92:
Bengtson, Angela M; Pence, Brian W; Eaton, Ellen F et al. (2018) Patterns of efavirenz use as first-line antiretroviral therapy in the United States: 1999-2015. Antivir Ther 23:363-372
Dubé, Karine; Gianella, Sara; Concha-Garcia, Susan et al. (2018) Ethical considerations for HIV cure-related research at the end of life. BMC Med Ethics 19:83
Mittal, María Luisa; Bazzi, Angela Robertson; Rangel, María Gudelia et al. (2018) 'He's not my pimp': toward an understanding of intimate male partner involvement in female sex work at the Mexico-US border. Cult Health Sex 20:961-975
Letendre, Scott; Bharti, Ajay; Perez-Valero, Ignacio et al. (2018) Higher Anti-Cytomegalovirus Immunoglobulin G Concentrations Are Associated With Worse Neurocognitive Performance During Suppressive Antiretroviral Therapy. Clin Infect Dis 67:770-777
Basova, Liana; Najera, Julia A; Bortell, Nikki et al. (2018) Dopamine and its receptors play a role in the modulation of CCR5 expression in innate immune cells following exposure to Methamphetamine: Implications to HIV infection. PLoS One 13:e0199861
Eren, Kemal; Murrell, Ben (2018) RIFRAF: a frame-resolving consensus algorithm. Bioinformatics 34:3817-3824
de Almeida, Sérgio Monteiro; Ribeiro, Clea E; Rotta, Indianara et al. (2018) Biomarkers of neuronal injury and amyloid metabolism in the cerebrospinal fluid of patients infected with HIV-1 subtypes B and C. J Neurovirol 24:28-40
Martin, Thomas C S; Rauch, Andri; Salazar-Vizcaya, Luisa et al. (2018) Understanding and Addressing Hepatitis C Virus Reinfection Among Men Who Have Sex with Men. Infect Dis Clin North Am 32:395-405
Jenks, Jeffrey D; Hoenigl, Martin (2018) Treatment of Aspergillosis. J Fungi (Basel) 4:

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