The Molecular Biology Core Facility at CWRU has operated for almost 8 years, providing state-of-the-art scientific capabilities to the research community, especially where expensive instrumentation or unique technical capabilities are required. To this end, the Core Facility has acquired a variety of instruments, the heart of which is 2 DNA synthesizers and a protein microsequencer. Based upon current limitations within the CWRU community, it is proposed that the Core Facility be expanded to provide the following: peptide synthesis, DNA sequencing, and large scale (up to 20 liters) fermentation of mammalian and microbial cultures. The necessity for these capabilities research projects as well as new investigators added to the CWRU faculty in the last two years. Funds are requested for the purchase of (i), a DNA sequencer (ii), a large scale fermenter and centrifuge for microbial fermentation and (iii), mammalian fermentation equipment. In addition, funds are requested to replace an eight year old DNA synthesizer. This replacement will allow for larger numbers of oligonucleotides to be prepared and will greatly upgrade both the reliability and capability of the synthesis of DNA oligonucleotides with or without non-standard nucleotides. In addition to instrumentation, funds are requested for partial support of three research technicians, associated with DNA sequencing, peptide synthesis (a peptide synthesizer exists in the Core Facility and is available to the CWRU community, however, past demand had not been sufficient to warrant hiring a technician for this effort) and bio-fermentation. These individuals will be associated with the unique demands of just a single instrument which will involve both reparative and analytical functions. Salary support of technical staff will gradually decrease throughout the funding period, and will be recovered by user fee. Calculation of user fees reflects the cost of supplies, salary support and annual maintenance contracts (due to the complex electronics of the instrument within the Core Facility, all are routinely covered by extended maintenance agreements with the manufacturer, although in some instances the appropriate individual may do trouble-shooting when a problem arises). The Core Facility has been operational for the last eight years and is familiar with the establishment of users fees. It has been the practice of the Core Facility to recover costs only as they are associated with each service and to not have any service supported by another.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Center Core Grants (P30)
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Case Western Reserve University
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Sahmoudi, Karima; Abbassi, Hassan; Bouklata, Nada et al. (2018) Immune activation and regulatory T cells in Mycobacterium tuberculosis infected lymph nodes. BMC Immunol 19:33
Webel, Allison R; Moore, Shirley M; Longenecker, Chris T et al. (2018) Randomized Controlled Trial of the SystemCHANGE Intervention on Behaviors Related to Cardiovascular Risk in HIV+ Adults. J Acquir Immune Defic Syndr 78:23-33
Altekruse, Sean F; Shiels, Meredith S; Modur, Sharada P et al. (2018) Cancer burden attributable to cigarette smoking among HIV-infected people in North America. AIDS 32:513-521
Eckard, Allison Ross; O'Riordan, Mary Ann; Rosebush, Julia C et al. (2018) Vitamin D supplementation decreases immune activation and exhaustion in HIV-1-infected youth. Antivir Ther 23:315-324
Webel, Allison R; Perazzo, Joseph; Longenecker, Christopher T et al. (2018) The Influence of Exercise on Cardiovascular Health in Sedentary Adults With Human Immunodeficiency Virus. J Cardiovasc Nurs 33:239-247
Yan, Junpeng; Shun, Ming-Chieh; Hao, Caili et al. (2018) HIV-1 Vpr Reprograms CLR4DCAF1 E3 Ubiquitin Ligase to Antagonize Exonuclease 1-Mediated Restriction of HIV-1 Infection. MBio 9:
Silver, Nicholas; Paynter, Mary; McAllister, Georgina et al. (2018) Characterization of minority HIV-1 drug resistant variants in the United Kingdom following the verification of a deep sequencing-based HIV-1 genotyping and tropism assay. AIDS Res Ther 15:18
Jiang, Wei; Luo, Zhenwu; Martin, Lisa et al. (2018) Drug Use is Associated with Anti-CD4 IgG-mediated CD4+ T Cell Death and Poor CD4+ T Cell Recovery in Viral-suppressive HIV-infected Individuals Under Antiretroviral Therapy. Curr HIV Res 16:143-150
Martinez, Leonardo; Handel, Andreas; Shen, Ye et al. (2018) A Prospective Validation of a Clinical Algorithm to Detect Tuberculosis in Child Contacts. Am J Respir Crit Care Med 197:1214-1216
Fitzgerald, Wendy; Freeman, Michael L; Lederman, Michael M et al. (2018) A System of Cytokines Encapsulated in ExtraCellular Vesicles. Sci Rep 8:8973

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