The overall objectives of UMass CFAR Molecular Virology Core facility are: (1) to maintain and continuouslymonitor the safety operations of a BSL3- and a BSL2-containment facility that will support a safe andbiohazard-free environment for work with pathogenic primate immunodeficiency viruses; (2) to provideresources and services to participating CFAR investigators for their ongoing virus-related projects; and (3) tofunction as a central site where collaborations amongst UMass CFAR investigators is fostered. The Coreservices include: (1) assays for isolation, titration, phenotypic and genotypic characterization of virus; (2)providing a centralized source and repository for primary T-cells, macrophages & T-cell lines; and (3)molecular detection/diagnostic assays including qualitative and quantitative RNA and DNA-PCR for viralgenomes & state of the art, cutting edge LightCycler-based PCR assays for real-time detection andquantitation of HIV-1. The Molecular Virology Core facility, in conjunction with UMass CFAR-Clinical andMolecular Biology Core facilities, uphold the mission of UMass CFAR by providing standardized andcentralized routine research techniques and reagents; reducing expenditure by eliminating duplication ofeffort & by efficient use of personnel; and fostering new collaborative projects and grants. The MolecularVirology Core is also committed to provide educational & training opportunities to CFAR investigators andstudents/fellows in working within BSL3 containment facility, in developing new molecular virology assays, inBSL3 Certification, and in PCR Certification. Details of the Molecular Virology Core facility, its location, itsfunctions, the services provided are all available on the UMass CFAR Molecular Virology Core website(www.umassmed.edu/cfar/viroloavcore).
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