The CFAR Molecular Core will provide CFAR investigators with the molecular biological regents and tools necessary to pursue their research by serving as a repository for expression vectors, antibodies and antisera, cell lines, and viral vectors. In addition to its role as a reagent repository, the Molecular Core will provide advice and expertise to investigators on such topics as the cloning and manipulation of genes and the functional analysis of viral proteins such as Env. Importantly, the Molecular Core will interface closely with the Virology Core. Viral genes will be isolated from primary viruses that are of interest to CFAR investigators and that are provided by the Virology Core, and placed into appropriate vectors. High level expression vectors will then be provided, when requested, to the Structural Core for large-scale production of gene products. The Molecular Core will also interact directly with the Clinical Core by genotyping patient samples for polymorphisms associated with altered disease progression. Thus, by consolidating and making available important reagents, by providing technical advance, by genotyping patient samples, and though the construction of expression systems the Molecular Core will facilitate interactions between the Virology, Clinical, and Structural Cores and will assist investigators in their research efforts. In addition, the Molecular Core will help foster collaborations and help draw new and established investigators into HIV/SIV research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI045008-02
Application #
6327582
Study Section
Project Start
2000-07-15
Project End
2001-06-30
Budget Start
Budget End
Support Year
2
Fiscal Year
2000
Total Cost
$165,421
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Page, Kathleen R; Grieb, Suzanne Dolwick; Nieves-Lugo, Karen et al. (2018) Enhanced immigration enforcement in the USA and the transnational continuity of HIV care for Latin American immigrants in deportation proceedings. Lancet HIV 5:e597-e604
Costea, Paul I; Hildebrand, Falk; Arumugam, Manimozhiyan et al. (2018) Enterotypes in the landscape of gut microbial community composition. Nat Microbiol 3:8-16
Opondo, Philip R; Ho-Foster, Ari R; Ayugi, James et al. (2018) HIV Prevalence Among Hospitalized Patients at the Main Psychiatric Referral Hospital in Botswana. AIDS Behav 22:1503-1516
Merlin, Jessica S; Long, Dustin; Becker, William C et al. (2018) Brief Report: The Association of Chronic Pain and Long-Term Opioid Therapy With HIV Treatment Outcomes. J Acquir Immune Defic Syndr 79:77-82
Clarke, Erik L; Lauder, Abigail P; Hofstaedter, Casey E et al. (2018) Microbial Lineages in Sarcoidosis. A Metagenomic Analysis Tailored for Low-Microbial Content Samples. Am J Respir Crit Care Med 197:225-234
Venuto, Charles S; Lim, Jihoon; Messing, Susan et al. (2018) Inflammation investigated as a source of pharmacokinetic variability of atazanavir in AIDS Clinical Trials Group protocol A5224s. Antivir Ther 23:345-351
Meyers, Kathrine; Rodriguez, Kristina; Brill, Atrina L et al. (2018) Lessons for Patient Education Around Long-Acting Injectable PrEP: Findings from a Mixed-Method Study of Phase II Trial Participants. AIDS Behav 22:1209-1216
Barbian, Hannah J; Connell, Andrew Jesse; Avitto, Alexa N et al. (2018) CHIIMP: An automated high-throughput microsatellite genotyping platform reveals greater allelic diversity in wild chimpanzees. Ecol Evol 8:7946-7963
Barbian, Hannah J; Li, Yingying; Ramirez, Miguel et al. (2018) Destabilization of the gut microbiome marks the end-stage of simian immunodeficiency virus infection in wild chimpanzees. Am J Primatol 80:
Abdel-Mohsen, Mohamed; Kuri-Cervantes, Leticia; Grau-Exposito, Judith et al. (2018) CD32 is expressed on cells with transcriptionally active HIV but does not enrich for HIV DNA in resting T cells. Sci Transl Med 10:

Showing the most recent 10 out of 775 publications