The goal of the IMMUNOLOGY AND PATHOLOGY CORE is to provide a centralized facility for performing a range of assays and services that facilitate the functional and anatomical analysis of cells and tissues by CFAR investigators. This facility provides the biosafety containment that is necessary for these analyses. Services provided by this core include RNA and protein analyses for cytokines, chemokines and growth factors, as well as tissue fixation, slide preparation and histopathology using microscopic examination, immunohistochemical and in situ hybridization techniques. We will soon offer additional cutting edge technology by providing use of an inverted fluorescence microscope to analyze HIV infected living cells and unfixed tissues. This core provides a key service, as an understanding of the mechanisms that mediate HIV and opportunistic infection and tissue responses requires an analysis of the factors being elaborated by and the phenotypes of infected cells as well as the response to infection by uninfected resident cells. Therefore, access to these services greatly supports the diverse research efforts of investigators at Einstein and Montefiore aimed at understanding the pathogenesis of infection with HIV-1 and AIDS-associated pathogens.
Aim 1. To provide investigators with the research infrastructure that is necessary to analyze the immune responses stimulated by HIV and AIDS-associated pathogens by measuring cytokine, chemokine and other soluble mediator, RNA, and protein levels in the appropriate biosafety containment.
Aim 2. To provide investigators with a facility using appropriate biohazard containment that is capable of examining the effects of infection with HIV and AIDS-associated pathogens on tissues by characterizing morphological, molecular and phenotypical changes using histological, immunohistochemical, in situ hybridization analyses and live cell imaging.
Aim 3. To train CFAR investigators in the performance of techniques that are extant in the Core and to assist investigators in the development of new immunological assays and pathological techniques that are required for the accomplishment of their research projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
3P30AI051519-10S1
Application #
8628354
Study Section
Special Emphasis Panel (ZAI1-EC-A)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
10
Fiscal Year
2013
Total Cost
$200,035
Indirect Cost
$71,003
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Vilchèze, Catherine; Kim, John; Jacobs Jr, William R (2018) Vitamin C Potentiates the Killing of Mycobacterium tuberculosis by the First-Line Tuberculosis Drugs Isoniazid and Rifampin in Mice. Antimicrob Agents Chemother 62:
Patel, Viraj V; Ginsburg, Zoë; Golub, Sarit A et al. (2018) Empowering With PrEP (E-PrEP), a Peer-Led Social Media-Based Intervention to Facilitate HIV Preexposure Prophylaxis Adoption Among Young Black and Latinx Gay and Bisexual Men: Protocol for a Cluster Randomized Controlled Trial. JMIR Res Protoc 7:e11375
Lankowski, Alexander J; Bien-Gund, Cedric H; Patel, Viraj V et al. (2018) PrEP in the Real World: Predictors of 6-Month Retention in a Diverse Urban Cohort. AIDS Behav :
Kirichenko, Tatiana V; Myasoedova, Veronika A; Shimonova, Tatiana E et al. (2018) Atherosclerosis in subjects newly diagnosed with human immunodeficiency virus infection. Biosci Rep 38:
Auld, Sara C; Shah, N Sarita; Mathema, Barun et al. (2018) Extensively drug-resistant tuberculosis in South Africa: genomic evidence supporting transmission in communities. Eur Respir J 52:
Harbut, Michael B; Yang, Baiyuan; Liu, Renhe et al. (2018) Small Molecules Targeting Mycobacterium tuberculosis Type II NADH Dehydrogenase Exhibit Antimycobacterial Activity. Angew Chem Int Ed Engl 57:3478-3482
Patel, Viraj V; Dange, Alpana; Rawat, Shruta et al. (2018) Barriers to HIV Testing Among Men Who Have Sex With Men in India Reached Online: Implications for Interventions. J Acquir Immune Defic Syndr 78:e30-e34
Shah, N Sarita; Auld, Sara C; Brust, James C M et al. (2017) Transmission of Extensively Drug-Resistant Tuberculosis in South Africa. N Engl J Med 376:243-253
Heo, Moonseong; Litwin, Alain H; Blackstock, Oni et al. (2017) Sample size determinations for group-based randomized clinical trials with different levels of data hierarchy between experimental and control arms. Stat Methods Med Res 26:399-413
Felsen, Uriel R; Cunningham, Chinazo O; Heo, Moonseong et al. (2017) Expanded HIV Testing Strategy Leveraging the Electronic Medical Record Uncovers Undiagnosed Infection Among Hospitalized Patients. J Acquir Immune Defic Syndr 75:27-34

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