Abstract

Acquisition, preparation, and culturing of tissue and cells are a critical, time-consuming, and expensive aspect of skin-oriented research. The Tissue Culture Core will serve as an efficient, systematic, and cost-effective system dedicated to the procurement, processing, and culture of skin- derived tissue and cells. Five major services will be provided: 1) reliable source of skin from human cadavers, newborn foreskin, and mice skin, 2) purification of epidermal cells (keratinocytes, Langerhans cells, dendritic epidermal T cells) and dermal cells (fibroblasts, and endothelial cells), 3) cell cultures of these different cell types, 4) purchase and preparation of tissue culture reagents in bulk quantities, and 5) quality control to monitor for contamination and to insure the purity of cell populations. These services should considerably reduce the time and expense devoted to skin-related projects, and will provide Core Investigators with a higher quality of skin-derived tissues and cells.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR041940-03
Application #
3748012
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Acharya, Asha; Baek, Seung Tae; Banfi, Serena et al. (2011) Efficient inducible Cre-mediated recombination in Tcf21 cell lineages in the heart and kidney. Genesis 49:870-7
Straud, Sarah; Zubovych, Iryna; De Brabander, Jef K et al. (2010) Inhibition of iron uptake is responsible for differential sensitivity to V-ATPase inhibitors in several cancer cell lines. PLoS One 5:e11629
Chapman, Shelby L; Sicot, F-X; Davis, Elaine C et al. (2010) Fibulin-2 and fibulin-5 cooperatively function to form the internal elastic lamina and protect from vascular injury. Arterioscler Thromb Vasc Biol 30:68-74
Yanagisawa, Hiromi; Davis, Elaine C (2010) Unraveling the mechanism of elastic fiber assembly: The roles of short fibulins. Int J Biochem Cell Biol 42:1084-93
Choi, Jiwon; Bergdahl, Andreas; Zheng, Qian et al. (2009) Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly. Matrix Biol 28:211-20
Jaeckle Santos, Lane J; Xing, Chao; Barnes, Robert B et al. (2008) Refined mapping of X-linked reticulate pigmentary disorder and sequencing of candidate genes. Hum Genet 123:469-76
Takayama, Yoshiharu; May, Petra; Anderson, Richard G W et al. (2005) Low density lipoprotein receptor-related protein 1 (LRP1) controls endocytosis and c-CBL-mediated ubiquitination of the platelet-derived growth factor receptor beta (PDGFR beta). J Biol Chem 280:18504-10
Wang, Huixia; He, Xuming; Band, Mark et al. (2005) A study of inter-lab and inter-platform agreement of DNA microarray data. BMC Genomics 6:71
Sinha, Suwan K; Zachariah, Sunny; Quinones, Herson I et al. (2002) Role of TRAF3 and -6 in the activation of the NF-kappa B and JNK pathways by X-linked ectodermal dysplasia receptor. J Biol Chem 277:44953-61
Sellati, T J; Waldrop, S L; Salazar, J C et al. (2001) The cutaneous response in humans to Treponema pallidum lipoprotein analogues involves cellular elements of both innate and adaptive immunity. J Immunol 166:4131-40

Showing the most recent 10 out of 56 publications