Abstract

I propose to develop a highly efficient system for expression of proteins in the keratinocytes of skin. Such system will have two important uses. One is to tolerize the host against a particular antigen. We have recently shown that restricting antigen expression to the keratinocytes in vivo decreases the immune response to subsequent exposure to the same antigen. Improving the expression system should allow extension of this concept to allergy and autoimmunity. The second is that such a system could be used for gene therapy of skin disorders.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR041940-11
Application #
6602471
Study Section
Special Emphasis Panel (ZAR1)
Project Start
2002-06-01
Project End
2006-05-31
Budget Start
Budget End
Support Year
11
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Acharya, Asha; Baek, Seung Tae; Banfi, Serena et al. (2011) Efficient inducible Cre-mediated recombination in Tcf21 cell lineages in the heart and kidney. Genesis 49:870-7
Straud, Sarah; Zubovych, Iryna; De Brabander, Jef K et al. (2010) Inhibition of iron uptake is responsible for differential sensitivity to V-ATPase inhibitors in several cancer cell lines. PLoS One 5:e11629
Chapman, Shelby L; Sicot, F-X; Davis, Elaine C et al. (2010) Fibulin-2 and fibulin-5 cooperatively function to form the internal elastic lamina and protect from vascular injury. Arterioscler Thromb Vasc Biol 30:68-74
Yanagisawa, Hiromi; Davis, Elaine C (2010) Unraveling the mechanism of elastic fiber assembly: The roles of short fibulins. Int J Biochem Cell Biol 42:1084-93
Choi, Jiwon; Bergdahl, Andreas; Zheng, Qian et al. (2009) Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly. Matrix Biol 28:211-20
Jaeckle Santos, Lane J; Xing, Chao; Barnes, Robert B et al. (2008) Refined mapping of X-linked reticulate pigmentary disorder and sequencing of candidate genes. Hum Genet 123:469-76
Takayama, Yoshiharu; May, Petra; Anderson, Richard G W et al. (2005) Low density lipoprotein receptor-related protein 1 (LRP1) controls endocytosis and c-CBL-mediated ubiquitination of the platelet-derived growth factor receptor beta (PDGFR beta). J Biol Chem 280:18504-10
Wang, Huixia; He, Xuming; Band, Mark et al. (2005) A study of inter-lab and inter-platform agreement of DNA microarray data. BMC Genomics 6:71
Sinha, Suwan K; Zachariah, Sunny; Quinones, Herson I et al. (2002) Role of TRAF3 and -6 in the activation of the NF-kappa B and JNK pathways by X-linked ectodermal dysplasia receptor. J Biol Chem 277:44953-61
Sellati, T J; Waldrop, S L; Salazar, J C et al. (2001) The cutaneous response in humans to Treponema pallidum lipoprotein analogues involves cellular elements of both innate and adaptive immunity. J Immunol 166:4131-40

Showing the most recent 10 out of 56 publications