The purpose of the molecular biology core will be facilitate training and utilization of molecular biology techniques among investigators in the Vanderbilt SDRC. The facilities and expertise of this Core will be available for all investigators who need assistance with quality control or analysis of critical molecular biology data needing independent verification. Core facilities will include not only the molecular equipment necessary to perform each of these techniques, but also an experienced staff for consultation on experimental design, demonstration of crucial techniques which might be new to investigators, and consultation on data interpretation. Specifically, we will assist and coordinate efforts to detect, quantitate and isolate novel regulatory and structural skin associated genes; coordinate and assist in the characterization of noel genes; assist in the functional analysis of novel genes: provide consultation and training for SDRC investigators and the pilot and feasibility projects; and maintain commonly used skin specific reagents, such as a library of RNA from human and mouse skin tissues; normal, diseased, and genetically altered. A core service for the design and cloning of new vectors/expression constructs, preparation of plasmid DNA, subcloning, and DNA sequence verification will be available. We will also provide consultation and help with nuclear run on analyses, in situ hybridization, and RNAse protection assays. Computerized analysis of sequence data will be available through the Genebank programs, BLAST, and the Vanderbilt Center VAX. The provision of these services should facilitate studies into the etiology of skin diseases and encourage clinical and basic scientists to utilize the clinical material and animal models available at Vanderbilt to further dermatological research.
| Russell, Shirley B; Smith, Joan C; Huang, Minjun et al. (2015) Pleiotropic Effects of Immune Responses Explain Variation in the Prevalence of Fibroproliferative Diseases. PLoS Genet 11:e1005568 |
| Velez Edwards, Digna R; Tsosie, Krystal S; Williams, Scott M et al. (2014) Admixture mapping identifies a locus at 15q21.2-22.3 associated with keloid formation in African Americans. Hum Genet 133:1513-23 |
| Duncan, F Jason; Silva, Kathleen A; Johnson, Charles J et al. (2013) Endogenous retinoids in the pathogenesis of alopecia areata. J Invest Dermatol 133:334-43 |
| Takahashi, Keiko; Mernaugh, Raymond L; Friedman, David B et al. (2012) Thrombospondin-1 acts as a ligand for CD148 tyrosine phosphatase. Proc Natl Acad Sci U S A 109:1985-90 |
| Jandova, Jana; Eshaghian, Alex; Shi, Mingjian et al. (2012) Identification of an mtDNA mutation hot spot in UV-induced mouse skin tumors producing altered cellular biochemistry. J Invest Dermatol 132:421-8 |
| Jandova, Jana; Shi, Mingjian; Norman, Kimberly G et al. (2012) Somatic alterations in mitochondrial DNA produce changes in cell growth and metabolism supporting a tumorigenic phenotype. Biochim Biophys Acta 1822:293-300 |
| Sundberg, J P; Taylor, D; Lorch, G et al. (2011) Primary follicular dystrophy with scarring dermatitis in C57BL/6 mouse substrains resembles central centrifugal cicatricial alopecia in humans. Vet Pathol 48:513-24 |
| Harries, M J; Sun, J; Paus, R et al. (2010) Management of alopecia areata. BMJ 341:c3671 |
| Yang, Jinming; Splittgerber, Ryan; Yull, Fiona E et al. (2010) Conditional ablation of Ikkb inhibits melanoma tumor development in mice. J Clin Invest 120:2563-74 |
| Russell, Shirley B; Russell, James D; Trupin, Kathryn M et al. (2010) Epigenetically altered wound healing in keloid fibroblasts. J Invest Dermatol 130:2489-96 |
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