Abstract

The relevance of stem cells to cutaneous biology is only now beginning to be appreciated. Evidence now points to a pivotal role for stem cells in normal skin homeostasis, regeneration after injury, and in development of cutaneous neoplasms. Only now are protocols being developed for isolation and in vitro propagation of circulating stem cells being developed and characterized. The proposal will develop tools needed to routinely isolate and propagate circulating stem cells that are committed to endothelial cell differentiation. The function of these cells and their roles in inflammatory, regenerative and neoplastic processes in the skin is only now beginning to be appreciated. The tools and data to be developed in this proposal will allow for submission of a grant proposal to multiple outside sources.
Specific aims : 1. To establish techniques for the growth of endothelial cells from peripheral blood stem cells in humans and to characterize the cells in regards to specific endothelial cell markers and growth characteristics. 2. To establish techniques for the growth of endothelial cells from bone marrow, fetal liver, embryonic stem cells, or peripheral blood stem cells in mice and to characterize the cells in regards to specific endothelial cell markers and growth characteristics.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR042687-08
Application #
6463599
Study Section
Special Emphasis Panel (ZAR1)
Project Start
1994-03-01
Project End
2004-04-30
Budget Start
Budget End
Support Year
8
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Emory University
Department
Type
DUNS #
042250712
City
Atlanta
State
GA
Country
United States
Zip Code
30322

  Publications

Pleniceanu, Oren; Shukrun, Racheli; Omer, Dorit et al. (2017) Peroxisome proliferator-activated receptor gamma (PPAR?) is central to the initiation and propagation of human angiomyolipoma, suggesting its potential as a therapeutic target EMBO Mol Med 9:508-530
Arbiser, Jack L; Bonner, Michael Y; Gilbert, Linda C (2017) Targeting the duality of cancer. NPJ Precis Oncol 1:
Díaz, Begoña; Ostapoff, Katherine T; Toombs, Jason E et al. (2016) Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model. Oncotarget 7:51569-51580
Laidlaw, Kamilla M E; Berhan, Samuel; Liu, Suhu et al. (2016) Cooperation of imipramine blue and tyrosine kinase blockade demonstrates activity against chronic myeloid leukemia. Oncotarget 7:51651-51664
Bhandarkar, Sulochana S; Lanka, Padmavathy; Lanka, Lakshmana Rao et al. (2016) Tuberculosis verrucosa cutis lesions exhibit a greater microvessel count than lupus vulgaris lesions. Exp Dermatol 25:479-80
Costa, Adilson; Bonner, Michael Yi; Arbiser, Jack L (2016) Use of Polyphenolic Compounds in Dermatologic Oncology. Am J Clin Dermatol 17:369-85
Bonner, Michael Y; Karlsson, Isabella; Rodolfo, Monica et al. (2016) Honokiol bis-dichloroacetate (Honokiol DCA) demonstrates activity in vemurafenib-resistant melanoma in vivo. Oncotarget 7:12857-68
Arbiser, Jack L; Bonner, Michael Y (2016) Seborrheic Keratoses: The Rodney Dangerfield of Skin lesions, and Why They Should Get Our Respect. J Invest Dermatol 136:564-566
Arbiser, Jack L (2014) PHIPing out: a genetic basis for tumor ulceration. J Invest Dermatol 134:600-602
Spence-Shishido, Allyson; Carr, Christopher; Bonner, Michael Y et al. (2013) In vivo Gram staining of tinea versicolor. JAMA Dermatol 149:991-2

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