Abstract

The incidence of malignant melanoma is increasing worldwide at rates that have been reported to be between 3 and 7%. In the United States, the incidence of melanoma is rising faster than any other malignancy. The overall goal of this project is to test the feasibility of using of Surface-Enhanced Laser Desorption/Ionization (SELDI) in melanoma biomarker discovery. The ultimate goal of the project is to develop proteomic approaches to the early detection of melanoma which can be utilized for screening. prognostication, selection of therapy, identification of potential targets for immunotherapy and the measurements of responses to therapy. The approach involves making no a priori assumptions regarding the nature of the proteins that may be useful as markers of melanoma but rather provides a high throughput system for analyzing the entire sample, in this project, the blood.
The specific aims are as follows: 1. To determine which combination of conditions are most suitable for screening protein peaks in plasma. 2. To perform a preliminary evaluation of 25 patient and 25 control sera.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR042689-08
Application #
6480559
Study Section
Special Emphasis Panel (ZAR1)
Project Start
1994-04-01
Project End
2004-03-31
Budget Start
Budget End
Support Year
8
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Tian, Tian; Jin, Michelle Qiushuang; Dubin, Krista et al. (2017) IL-1R Type 1-Deficient Mice Demonstrate an Impaired Host Immune Response against Cutaneous Vaccinia Virus Infection. J Immunol 198:4341-4351
Pan, Youdong; Tian, Tian; Park, Chang Ook et al. (2017) Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism. Nature 543:252-256
Volpicelli, Elgida R; Lezcano, Cecilia; Zhan, Qian et al. (2014) The multidrug-resistance transporter ABCB5 is expressed in human placenta. Int J Gynecol Pathol 33:45-51
Dowlatshahi, Mitra; Huang, Victor; Gehad, Ahmed E et al. (2013) Tumor-specific T cells in human Merkel cell carcinomas: a possible role for Tregs and T-cell exhaustion in reducing T-cell responses. J Invest Dermatol 133:1879-89
Guenova, Emmanuella; Watanabe, Rei; Teague, Jessica E et al. (2013) TH2 cytokines from malignant cells suppress TH1 responses and enforce a global TH2 bias in leukemic cutaneous T-cell lymphoma. Clin Cancer Res 19:3755-63
Majewska-Szczepanik, Monika; Paust, Silke; von Andrian, Ulrich H et al. (2013) Natural killer cell-mediated contact sensitivity develops rapidly and depends on interferon-?, interferon-? and interleukin-12. Immunology 140:98-110
Zadran, Sohila; McMickle, Robert; Shackelford, David et al. (2013) Monitoring extra-vascular migratory metastasis (EVMM) of migrating cancer cells using an in vitro co-culture system. Protoc exch 2013:
Burkhardt, Ute E; Hainz, Ursula; Stevenson, Kristen et al. (2013) Autologous CLL cell vaccination early after transplant induces leukemia-specific T cells. J Clin Invest 123:3756-65
Seneschal, Julien; Clark, Rachael A; Gehad, Ahmed et al. (2012) Human epidermal Langerhans cells maintain immune homeostasis in skin by activating skin resident regulatory T cells. Immunity 36:873-84
Girouard, Sasha D; Laga, Alvaro C; Mihm, Martin C et al. (2012) SOX2 contributes to melanoma cell invasion. Lab Invest 92:362-70

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