Abstract

The Tissue, Cell and DNA Processing Core has been established to meet the continuing needs of a group of currently funded NIH investigators, as well as the needs of certain of the Pilot and Feasibility Studies. The functions of this Core include (1) primary culture from tissue of individuals with genetic diseases (fibroblasts, keratinocytes, human thyroid cells, human mammary epithelial cells), with cryo-preservation. (2) expanded cell culture of primary cells, as well as EBV-transformed cell lines, with cryo-preservation. (3) DNA and RNA extraction from tissues and cells, with DNA and RNA 'storage. (4) EBV-transformation of human B lymphocytes, with cryo-preservation. (5) provision of training in cell culture and preservation, DNA and RNA extraction to Center investigators. As the central theme of this Skin Diseases Research Center is human genetics, it is likely that this Core will be heavily utilized by Center investigators as a Core facility that will preserve valuable human samples for research.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR044535-04
Application #
6345969
Study Section
Project Start
2000-07-01
Project End
2002-06-30
Budget Start
Budget End
Support Year
4
Fiscal Year
2000
Total Cost
$77,307
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Type
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032

  Publications

Shen, Yao; Stanislauskas, Milda; Li, Gen et al. (2017) Epigenetic and genetic dissections of UV-induced global gene dysregulation in skin cells through multi-omics analyses. Sci Rep 7:42646
Mathew, Grinu; Hannan, Abdul; Hertzler-Schaefer, Kristina et al. (2016) Targeting of Ras-mediated FGF signaling suppresses Pten-deficient skin tumor. Proc Natl Acad Sci U S A 113:13156-13161
Shen, Yao; Kim, Arianna L; Du, Rong et al. (2016) Transcriptome Analysis Identifies the Dysregulation of Ultraviolet Target Genes in Human Skin Cancers. PLoS One 11:e0163054
Dai, Zhenpeng; Xing, Luzhou; Cerise, Jane et al. (2016) CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata. J Immunol 197:1089-99
Abaci, Hasan E; Guo, Zongyou; Coffman, Abigail et al. (2016) Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC-Derived Endothelial Cells. Adv Healthc Mater 5:1800-7
Dainichi, Teruki; Hayden, Matthew S; Park, Sung-Gyoo et al. (2016) PDK1 Is a Regulator of Epidermal Differentiation that Activates and Organizes Asymmetric Cell Division. Cell Rep 15:1615-23
Johnson, Dylan; Mathur, Mohit C; Kobayashi, Tomoyoshi et al. (2016) The Cardiomyopathy Mutation, R146G Troponin I, Stabilizes the Intermediate ""C"" State of Regulated Actin under High- and Low-Free Ca(2+) Conditions. Biochemistry 55:4533-40
Shinkuma, Satoru; Guo, Zongyou; Christiano, Angela M (2016) Site-specific genome editing for correction of induced pluripotent stem cells derived from dominant dystrophic epidermolysis bullosa. Proc Natl Acad Sci U S A 113:5676-81
Kim, Arianna L; Back, Jung Ho; Zhu, Yucui et al. (2016) AKT1 Activation is Obligatory for Spontaneous BCC Tumor Growth in a Murine Model that Mimics Some Features of Basal Cell Nevus Syndrome. Cancer Prev Res (Phila) 9:794-802
Sun, Xiaoyun; Kim, Arianna; Nakatani, Masashi et al. (2016) Distinctive molecular responses to ultraviolet radiation between keratinocytes and melanocytes. Exp Dermatol 25:708-13

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