Abstract

Many cell types are involved in the inflammatory process in rheumatic diseases. Understanding the biology of these diseases will require dissecting out the contribution of each of these cell subpopulations. The Cell Isolation and Phenotyping Core will provide technical expertise, assistance, and equipment of a reasonable cost to investigators in the research base whose studies require cell analysis. The Core will also provide training in the proper user and interpretation of flow cytometry. Expertise and assistance will be provided by core personnel in the following areas: Cell surface phenotype analysis Intracellular cytokine analysis Cell cycle analysis DNA index analysis Apoptosis analysis Gene expression analysis (green fluorescent protein, beta- galactosidase) Calcium flux analysis Cell sorting In addition, the Core will facilitate the acquisition, analysis, and storage of flow cytometry data, maintain standards and quality control for flow cytometry procedures, and assist in the development of new techniques as needed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
1P30AR047363-01
Application #
6447615
Study Section
Special Emphasis Panel (ZAR1)
Project Start
2001-03-15
Project End
2006-02-28
Budget Start
Budget End
Support Year
1
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Cincinnati Children's Hospital Medical Center
Department
Type
DUNS #
071284913
City
Cincinnati
State
OH
Country
United States
Zip Code
45229

  Publications

Rydyznski, Carolyn E; Cranert, Stacey A; Zhou, Julian Q et al. (2018) Affinity Maturation Is Impaired by Natural Killer Cell Suppression of Germinal Centers. Cell Rep 24:3367-3373.e4
Carroll, Kaitlin R; Elfers, Eileen E; Stevens, Joseph J et al. (2018) Extending Remission and Reversing New-Onset Type 1 Diabetes by Targeted Ablation of Autoreactive T Cells. Diabetes 67:2319-2328
Goodman, Michael Aaron; Arumugam, Paritha; Pillis, Devin Marie et al. (2018) Foamy Virus Vector Carries a Strong Insulator in Its Long Terminal Repeat Which Reduces Its Genotoxic Potential. J Virol 92:
Hinks, Anne; Marion, Miranda C; Cobb, Joanna et al. (2018) Brief Report: The Genetic Profile of Rheumatoid Factor-Positive Polyarticular Juvenile Idiopathic Arthritis Resembles That of Adult Rheumatoid Arthritis. Arthritis Rheumatol 70:957-962
Gupta, Varsha; Tangpricha, Vin; Yow, Eric et al. (2018) Analysis of relationships between 25-hydroxyvitamin D, parathyroid hormone and cathelicidin with inflammation and cardiovascular risk in subjects with paediatric systemic lupus erythematosus: an Atherosclerosis Prevention in Paediatric Lupus Erythematosus Lupus Sci Med 5:e000255
Rochman, Yrina; Dienger-Stambaugh, Krista; Richgels, Phoebe K et al. (2018) TSLP signaling in CD4+ T cells programs a pathogenic T helper 2 cell state. Sci Signal 11:
McIntosh, Laura A; Marion, Miranda C; Sudman, Marc et al. (2017) Genome-Wide Association Meta-Analysis Reveals Novel Juvenile Idiopathic Arthritis Susceptibility Loci. Arthritis Rheumatol 69:2222-2232
McCauley, Heather A; Chevrier, Véronique; Birnbaum, Daniel et al. (2017) De-repression of the RAC activator ELMO1 in cancer stem cells drives progression of TGF?-deficient squamous cell carcinoma from transition zones. Elife 6:
Litosh, Vladislav A; Rochman, Mark; Rymer, Jeffrey K et al. (2017) Calpain-14 and its association with eosinophilic esophagitis. J Allergy Clin Immunol 139:1762-1771.e7
Lages, Celine S; Simmons, Julia; Maddox, Avery et al. (2017) The dendritic cell-T helper 17-macrophage axis controls cholangiocyte injury and disease progression in murine and human biliary atresia. Hepatology 65:174-188

Showing the most recent 10 out of 214 publications