Several cell types are involved in the inflammatory process in rheumatic diseases. Understanding thebiology of these diseases will require dissecting out the contribution of each of these cellsubpopulations. The Integrative Cell Phenotyping and Morphology Core will provide technicalexpertise, assistance and equipment at a reasonable cost to investigators in the research base whosestudies require single cell analysis. In this renewal, the former Cell Phenotyping Core has expanded itscapabilities to offer expertise in single cell analysis via pathology methodologies. The Core will providetraining in the proper use and interpretation of specific immunophenotyping methodologies includingflow cytometry, immunohistochemistry, in situ hybridization and histology. Expertise and assistance willbe provided by core personnel in the following areas: Cell surface phenotype analysis (flow-based and pathology-based); Morphological cell phenotype analysis (pathology based); Intracellular cytokine analysis (flow-based, and pathology-based); Cell proliferation analysis (flow-based); Cell cycle and DNA index analysis (flow-based); Cell apoptosis analysis (flow-based and pathology-based); Gene expression analysis (flow-based and pathology-based); Calcium flux analysis (flow-based); Cell sorting (flow-based); Antigen-specific T cell analysis (flow-based).In addition, the Core will facilitate the acquisition, analysis and storage of immunophenotyping data,maintain standards and quality control for immunophenotyping procedures, and assist in thedevelopment of new techniques as needed.
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