In the first cycle of this SDRC, the DNA/RNA Delivery Core was widely used by SDRC investigators involved in cutaneous biology studies. This Core has provided researchers with expression vectors, lenti/ retroviruses to overexpress or silence genes and to express reporters in skin cells. The Core also performed cell infections for researchers and provided training and consultations on viral DNA/RNA Delivery. Generation of lentiviruses expressing shRNA and MlRs have been the most highly sought services requested by SDRC members and Pilot and Feasibility projects. During past few years, the Core dramatically expanded the vector repository. The Core now offers a broad panel of vectors for expression of: (i) cDNA with single or dual promoters, polycistronic design, C/N terminal protein tags and fluorescent fusion proteins;(ii) stem-loop precursors of shRNA/MiR or functionally active mature RNAi oligos to silence gene/protein expression;(iii) reporters for cell tracking (with fluorescent or luminescent markers), studies of transcription factor (Luciferase reporters), and high throughput (HTP) screening. We anticipate further extension and diversification of the vector repository in the next five years, as well as provision to users of the most powerful technical approaches in the quickly progressing field of viral delivery. In collaboration with other SDRC and NU Cores, we developed viruses and protocols for special applications, such as infection of primary keratinocytes within raft cultures;in vitro and in vivo cell tracking;screening of chemical libraries;and production of cytokines and biologically active regulators. Individual consultations by the Core have been frequently requested by SDRC members to aid in technical aspects of their research projects. The expertise of the Directors and the Core specialist will continue to help investigators in evaluating the roles that their genes/proteins of interest play in skin. We will continue to ensure that services, training and troubleshooting skills provided by the Core, as well as vector and viral stocks libraries, lead to increased capabilities and cost-effectiveness for SDRC investigators, thus strengthening the existing collaborative ventures and attracting new researchers to the field of cutaneous biology.
The DNA/RNA Delivery Core provides SDRC members and P&F projects with the most advanced tools for delivery of genetic material into skin cells, as well as consultations and training to facilitate their studies. In addition, this Core will extend its library of frequently requested vectors and viruses to aid SDRC members in the translational aspects of their research and to enhance cutaneous biology research at NU.
|Chu, Lan H; Indramohan, Mohanalaxmi; Ratsimandresy, Rojo A et al. (2018) The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat Commun 9:996|
|Tsoi, Lam C; Yang, Jingjing; Liang, Yun et al. (2018) Transcriptional determinants of individualized inflammatory responses at anatomically separate sites. J Allergy Clin Immunol 141:805-808|
|Kaplan, Nihal; Ventrella, Rosa; Peng, Han et al. (2018) EphA2/Ephrin-A1 Mediate Corneal Epithelial Cell Compartmentalization via ADAM10 Regulation of EGFR Signaling. Invest Ophthalmol Vis Sci 59:393-406|
|Nekrasova, Oxana; Harmon, Robert M; Broussard, Joshua A et al. (2018) Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat Commun 9:1053|
|Kishibe, Mari; Griffin, Tina M; Goslawski, Melissa et al. (2018) Topical nicotinic receptor activation improves wound bacterial infection outcomes and TLR2-mediated inflammation in diabetic mouse wounds. Wound Repair Regen 26:403-412|
|Kam, Chen Yuan; Dubash, Adi D; Magistrati, Elisa et al. (2018) Desmoplakin maintains gap junctions by inhibiting Ras/MAPK and lysosomal degradation of connexin-43. J Cell Biol 217:3219-3235|
|North, Jeffrey P; Golovato, Justin; Vaske, Charles J et al. (2018) Cell of origin and mutation pattern define three clinically distinct classes of sebaceous carcinoma. Nat Commun 9:1894|
|Rübsam, Matthias; Broussard, Joshua A; Wickström, Sara A et al. (2018) Adherens Junctions and Desmosomes Coordinate Mechanics and Signaling to Orchestrate Tissue Morphogenesis and Function: An Evolutionary Perspective. Cold Spring Harb Perspect Biol 10:|
|Ratsimandresy, Rojo A; Chu, Lan H; Khare, Sonal et al. (2017) The PYRIN domain-only protein POP2 inhibits inflammasome priming and activation. Nat Commun 8:15556|
|Bagchi, Sreya; He, Ying; Zhang, Hong et al. (2017) CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice. J Clin Invest 127:2339-2352|
Showing the most recent 10 out of 102 publications