Abstract

Until recently, we supported three separate shared services that provided various methods for the qualitative or quantitative analysis of macromolecules, including Darkroom, Radioisotope Counting, and Flow Cytometry Facilities. In the last five years, we have taken advantage of new technologies in this area through the purchase of phosphorimagers and automated DNA sequencers. We have combined all of these resources into the Macromolecular Analysis Facility in this application because of their common objectives and to increase the efficiency of their operation. The goal of this shared service is to provide researchers in the Center with access to essential, well-maintained analytical instruments and training in their use. Individual investigators are responsible for sample preparation and, with the exception of DNA sequencing, the operation of the instrument.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA007175-35
Application #
6101418
Study Section
Project Start
1999-04-01
Project End
2000-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
35
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Thompson, Nancy E; Glaser, Bryan T; Foley, Katherine M et al. (2009) Minimal promoter systems reveal the importance of conserved residues in the B-finger of human transcription factor IIB. J Biol Chem 284:24754-66
Habig, Jeffrey W; Loeb, Daniel D (2006) Sequence identity of the direct repeats, DR1 and DR2, contributes to the discrimination between primer translocation and in situ priming during replication of the duck hepatitis B virus. J Mol Biol 364:32-43
Liu, Ning; Ji, Lin; Maguire, Megan L et al. (2004) cis-Acting sequences that contribute to the synthesis of relaxed-circular DNA of human hepatitis B virus. J Virol 78:642-9
Tessier, Charles R; Doyle, Glenn A; Clark, Brad A et al. (2004) Mammary tumor induction in transgenic mice expressing an RNA-binding protein. Cancer Res 64:209-14
Ostrow, Kristin M; Loeb, Daniel D (2004) Underrepresentation of the 3' region of the capsid pregenomic RNA of duck hepatitis B virus. J Virol 78:2179-86
Habig, Jeffrey W; Loeb, Daniel D (2003) Template switches during plus-strand DNA synthesis of duck hepatitis B virus are influenced by the base composition of the minus-strand terminal redundancy. J Virol 77:12412-20
Habig, Jeffrey W; Loeb, Daniel D (2003) The conformation of the 3' end of the minus-strand DNA makes multiple contributions to template switches during plus-strand DNA synthesis of duck hepatitis B virus. J Virol 77:12401-11
Bunger, Maureen K; Moran, Susan M; Glover, Edward et al. (2003) Resistance to 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity and abnormal liver development in mice carrying a mutation in the nuclear localization sequence of the aryl hydrocarbon receptor. J Biol Chem 278:17767-74
Liu, Ning; Tian, Ru; Loeb, Daniel D (2003) Base pairing among three cis-acting sequences contributes to template switching during hepadnavirus reverse transcription. Proc Natl Acad Sci U S A 100:1984-9
Habig, Jeffrey W; Loeb, Daniel D (2002) Small DNA hairpin negatively regulates in situ priming during duck hepatitis B virus reverse transcription. J Virol 76:980-9

Showing the most recent 10 out of 79 publications