The Flow Cytometry Core at MSKCC provides researchers at MSKCC with state of the art flow cytometry services, including cell sorting. These services include operator-assisted flow cytometry analysis on a CyAn and a FACSCaliburflow cytometer, self-operated flow analysis on two 5-color (upgraded) FACScans and a FACSCalibur, and operator-assisted cell sorting on one 4-laser MoFlo, one 3-laser MoFlo, and one 3-laser DiVa cell sorter. Extensive training in flow cytometry is offered, and a site license for Flowjo is administered by the core facility. An additional cell purification option, Miltenyi AutoMACS, is made available to all users. The availability of these services enables the researchers at MSKCC to isolate and characterize specific cellular subsets, and to analyze the effects of manipulations aimed at answering a multitude of scientific questions that arise within the context of cancer research. The core facility's services play in important role in enabling the researchers to ask specific questions regarding ever smaller subsets of cells, down to the single cell level. Many of these questions can only be answered when purified fractions of specific cell subsets can be identified and isolated, which is the domain of flow analysis and cell sorting.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA008748-46
Application #
8243720
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2011-01-01
Budget End
2011-12-31
Support Year
46
Fiscal Year
2011
Total Cost
$379,635
Indirect Cost
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Staehler, M; Motzer, R J; George, D J et al. (2018) Adjuvant sunitinib in patients with high-risk renal cell carcinoma: safety, therapy management, and patient-reported outcomes in the S-TRAC trial. Ann Oncol 29:2098-2104
Cybulska, Paulina; Goss, Cheryl; Tew, William P et al. (2018) Corrigendum to ""Indications for and complications of transfusion and the management of gynecologic malignancies"" [Gynecol. Oncol. 146 (2017) 416-426]. Gynecol Oncol 148:235
Giulino-Roth, Lisa; O'Donohue, Tara; Chen, Zhengming et al. (2018) Outcome of children and adolescents with relapsed Hodgkin lymphoma treated with high-dose therapy and autologous stem cell transplantation: the Memorial Sloan Kettering Cancer Center experience. Leuk Lymphoma 59:1861-1870
Kidwell, Kelley M; Postow, Michael A; Panageas, Katherine S (2018) Sequential, Multiple Assignment, Randomized Trial Designs in Immuno-oncology Research. Clin Cancer Res 24:730-736
Dean, Jamie; Wong, Kee; Gay, Hiram et al. (2018) Incorporating spatial dose metrics in machine learning-based normal tissue complication probability (NTCP) models of severe acute dysphagia resulting from head and neck radiotherapy. Clin Transl Radiat Oncol 8:27-39
Luo, Jun; Attard, Gerhardt; Balk, Steven P et al. (2018) Role of Androgen Receptor Variants in Prostate Cancer: Report from the 2017 Mission Androgen Receptor Variants Meeting. Eur Urol 73:715-723
Morales, K A; Arevalo-Perez, J; Peck, K K et al. (2018) Differentiating Atypical Hemangiomas and Metastatic Vertebral Lesions: The Role of T1-Weighted Dynamic Contrast-Enhanced MRI. AJNR Am J Neuroradiol 39:968-973
Lee, Sang Ho; Rimner, Andreas; Gelb, Emily et al. (2018) Correlation Between Tumor Metabolism and Semiquantitative Perfusion Magnetic Resonance Imaging Metrics in Non-Small Cell Lung Cancer. Int J Radiat Oncol Biol Phys 102:718-726
Ramaswamy, Kavitha; Forbes, Lauren; Minuesa, Gerard et al. (2018) Peptidomimetic blockade of MYB in acute myeloid leukemia. Nat Commun 9:110
Amin, Sadaf; Cook, Brandoch; Zhou, Ting et al. (2018) Discovery of a drug candidate for GLIS3-associated diabetes. Nat Commun 9:2681

Showing the most recent 10 out of 8799 publications