The primary goal of the X-ray Crystallography Core Facility is to provide access to state-of-the-art hardware, software and expertise to Cancer Center laboratories that use X-ray crystallography as a tool to address questions in their research programs. The Core provides and maintains In-house equipment and computational resources for data collection, processing and structure determination, implements a wide range of crystallographic and structure analysis software packages, participates in multi-institutional consortia for long-term regular access to synchrotron beamlines, and provides training and technical assistance to users of both the in-house and remote facilities. In addition, the Core Head collaborates with non-structural MSKCC groups on macromolecular crystallization and structure-determination projects, and also provides modeling expertise and guidance to those laboratories that can benefit from the use of available structures in the design and interpretation of experiments. Understanding the biological processes involved in tumorigenesis has increasingly benefitted from structural investigations that provide key functional and mechanistic Insights, as well as atomic-level details important for drug discovery. Many of the questions require the structure determination of large proteins, assemblies and macromolecular machines. Advances in protein expression technologies and nano-liter crystallization robots are making it possible to obtain hitherto inaccessible crystals. However, crystals of such complexes often present major experimental challenges due to small crystal size, large unit cells, poor order and limited diffraction. The X-ray Core alms to help address these limitations for MSKCC investigators by providing the latest generation in-house X-ray diffraction systems, by testing and implementing new methods, tools and software and disseminating the latest improvements, and by participating in multi-institutional consortia that not only build and operate synchrotron beamlines, but also carry out technology research and development that push the envelope of beamline capabilities. The X-Ray Crystallography Core has supported 8 investigators in the past year. During the past grant period the Core has contributed to 115 publications of researchers from 3 programs.

Public Health Relevance

Understanding the biological processes involved in tumorigenesis requires the understanding of the underlying structures of relevant proteins, genes and enzymes. The X-Ray Crystallography core provides services to MSKCC investigators interested in structure-function determinations.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA008748-48
Application #
8933521
Study Section
Subcommittee G - Education (NCI)
Program Officer
Shafik, Hasnaa
Project Start
2014-01-01
Project End
2018-12-31
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
48
Fiscal Year
2014
Total Cost
$271,886
Indirect Cost
$118,883
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Shamay, Yosi; Shah, Janki; I??k, Mehtap et al. (2018) Quantitative self-assembly prediction yields targeted nanomedicines. Nat Mater 17:361-368
Drilon, Alexander; Laetsch, Theodore W; Kummar, Shivaani et al. (2018) Efficacy of Larotrectinib in TRK Fusion-Positive Cancers in Adults and Children. N Engl J Med 378:731-739
Cracchiolo, Jennifer R; Xu, Bin; Migliacci, Jocelyn C et al. (2018) Patterns of recurrence in oral tongue cancer with perineural invasion. Head Neck 40:1287-1295
Sanchez, Elisa; Huse, Morgan (2018) Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist. J Vis Exp :
Brufsky, Adam M; Dickler, Maura N (2018) Estrogen Receptor-Positive Breast Cancer: Exploiting Signaling Pathways Implicated in Endocrine Resistance. Oncologist 23:528-539
Varughese, Tilly; Taur, Ying; Cohen, Nina et al. (2018) Serious Infections in Patients Receiving Ibrutinib for Treatment of Lymphoid Cancer. Clin Infect Dis 67:687-692
Pacheco, Sarai; Maldonado-Linares, Andros; Marcet-Ortega, Marina et al. (2018) ATR is required to complete meiotic recombination in mice. Nat Commun 9:2622
Marzouk, Karim; Tin, Amy; Liu, Nick et al. (2018) The natural history of large renal masses followed on observation. Urol Oncol 36:362.e17-362.e21
Shickh, Salma; Clausen, Marc; Mighton, Chloe et al. (2018) Evaluation of a decision aid for incidental genomic results, the Genomics ADvISER: protocol for a mixed methods randomised controlled trial. BMJ Open 8:e021876
Mobley, David L; Bannan, Caitlin C; Rizzi, Andrea et al. (2018) Escaping Atom Types in Force Fields Using Direct Chemical Perception. J Chem Theory Comput 14:6076-6092

Showing the most recent 10 out of 8799 publications