The Flow Cytometry (FC) Core offers centralized expertise and instrumentation in Flow Cytometry and Cell Sorting to investigators of the Cancer Center. The extensive user support includes a) assisting in planning, executing, and analyzing Flow Cytometry experiments; b) daily instrument setup, maintenance, quality control and performance monitoring; c) facilitating instrument access through implementation of adequate polices with an easy and reliable scheduling system; d) offering several software site licenses for high-end Flow data analysis; and e) extensive training and education options. Instrument based-services include operator-assisted cell sorting in five multiparametric FACSAria high-speed cell sorters, user-operated cell sorting in two multiparametric FACSAria cell sorters, and user-operated analysis in five high-end multiparametric analyzers (two LSRII and three LSR Fortessa) and a four-parameter FACSCalibur. Altogether, the availability of high-end instrumentation and highly qualified staff with experience provide investigators of the Center access to highly purified cell populations, and the possibility to characterize cell subsets involved in the processes of tumor development, which can ultimately lead to improved cancer diagnosis and therapy. The services provided by the FC Core have supported 239 investigators from over 140 laboratories in the last year. During the past grant period the efforts of the Core contributed to 843 publications of researchers from the 10 Programs. As an illustration of this support, a recent collaborative study among the laboratories of Drs. Sadelain (ET,CR), van den Brink (IT,CR), and Schietinger (IT) was facilitated by the Core?s expertise and instrumentation provided to analyze immunophenotypic markers in T cells, phopho-flow to analyze T cell signal transduction pathways, as well as state-of-the-art sorting services to isolate GFP-labeled CAR T cells from mouse transplants that were subsequently subjected to transcriptome profiling. The resulting publication of this study provides insight into mechanisms of allogeneic CD19 CAR T cells promoting anti-tumor activity in lymphomas.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee I - Transistion to Independence (NCI)
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Sloan-Kettering Institute for Cancer Research
New York
United States
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Malard, Florent; Labopin, Myriam; Cho, Christina et al. (2018) Ex vivo and in vivo T cell-depleted allogeneic stem cell transplantation in patients with acute myeloid leukemia in first complete remission resulted in similar overall survival: on behalf of the ALWP of the EBMT and the MSKCC. J Hematol Oncol 11:127
Meyer, Jan-Philip; Tully, Kathryn M; Jackson, James et al. (2018) Bioorthogonal Masking of Circulating Antibody-TCO Groups Using Tetrazine-Functionalized Dextran Polymers. Bioconjug Chem 29:538-545
McFarland, Daniel C; Shaffer, Kelly; Breitbart, William et al. (2018) C-reactive protein and its association with depression in patients receiving treatment for metastatic lung cancer. Cancer :
Lacouture, Mario E; Anadkat, Milan; Jatoi, Aminah et al. (2018) Dermatologic Toxicity Occurring During Anti-EGFR Monoclonal Inhibitor Therapy in Patients With Metastatic Colorectal Cancer: A Systematic Review. Clin Colorectal Cancer 17:85-96
Blumenthal, Gideon M; Bunn Jr, Paul A; Chaft, Jamie E et al. (2018) Current Status and Future Perspectives on Neoadjuvant Therapy in Lung Cancer. J Thorac Oncol 13:1818-1831
Shaffer, Kelly M; Nelson, Christian J; DuHamel, Katherine N (2018) Barriers to participation in a sexual health intervention for men following treatment for rectal and anal cancer. Psychooncology 27:1082-1085
Park, Kay J; Patel, Prusha; Linkov, Irina et al. (2018) Observations on the origin of ovarian cortical inclusion cysts in women undergoing risk-reducing salpingo-oophorectomy. Histopathology 72:766-776
Pianko, Matthew J; Goldberg, Aaron D; Lesokhin, Alexander M (2018) Clinical Development of PD-1 Blockade in Hematologic Malignancies. Cancer J 24:31-35
Navi, Babak B; Marshall, Randolph S; Bobrow, Dylan et al. (2018) Enoxaparin vs Aspirin in Patients With Cancer and Ischemic Stroke: The TEACH Pilot Randomized Clinical Trial. JAMA Neurol 75:379-381
Shah, Sona; Boucai, Laura (2018) Effect of Age on Response to Therapy and Mortality in Patients With Thyroid Cancer at High Risk of Recurrence. J Clin Endocrinol Metab 103:689-697

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