The Molecular Cytogenetics Core provides valuable scientific and technical support to MSK investigators exploring the role of chromosomal instability in cancer. This is accomplished through appropriate and efficient experiment design, custom multi-color DNA-FISH probe design and development, data analysis and interpretation, and support with manuscript/grant preparation. Currently, the Core provides 2 main categories of specialized service that operate at the single-cell level on a variety of human and animal model research samples: conventional karyotyping and fluorescence in situ hybridization (FISH). Conventional karyotyping is routinely performed to confirm cell line identity, screen targeted mouse ESC clones intended for blastocyst injection, monitor the stability of cultured stem cells over time, and measure chromosomal instability/heterogeneity as an experimental endpoint. Over the years, the Core has assembled a broad range of molecular cytogenetics resources (plasmids, BAC clones, individual whole chromosome painting probes), allowing the Core to provide FISH services at significantly lower cost. FISH-based assays are routinely employed to map genes or transgene integration sites, detect aneuploidy/chromosomal instability, co-detect DNA/protein and assess intra-tumoral heterogeneity; tests include custom probe development, locus-specific FISH, whole-chromosome painting (WCP), spectral karyotyping (SKY), Q-FISH, and Immuno-FISH. The Core is equipped with 5 modern Fluorescence imaging systems including 2 with a wide range of applications that can be fully or partially automated. The Core also provides training in tissue culture, karyotyping, and FISH. During the past grant period, the services and collaborative work provided by the Molecular Cytogenetics Core have supported the research of 84 investigators from 8 research program.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee I - Transistion to Independence (NCI)
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Sloan-Kettering Institute for Cancer Research
New York
United States
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Pacheco, Sarai; Maldonado-Linares, Andros; Marcet-Ortega, Marina et al. (2018) ATR is required to complete meiotic recombination in mice. Nat Commun 9:2622
Marzouk, Karim; Tin, Amy; Liu, Nick et al. (2018) The natural history of large renal masses followed on observation. Urol Oncol 36:362.e17-362.e21
Shickh, Salma; Clausen, Marc; Mighton, Chloe et al. (2018) Evaluation of a decision aid for incidental genomic results, the Genomics ADvISER: protocol for a mixed methods randomised controlled trial. BMJ Open 8:e021876
Mobley, David L; Bannan, Caitlin C; Rizzi, Andrea et al. (2018) Escaping Atom Types in Force Fields Using Direct Chemical Perception. J Chem Theory Comput 14:6076-6092
Latham, Alicia; Srinivasan, Preethi; Kemel, Yelena et al. (2018) Microsatellite Instability Is Associated With the Presence of Lynch Syndrome Pan-Cancer. J Clin Oncol :JCO1800283
Husain, Qasim; Gupta, Piyush; Tabar, Viviane et al. (2018) Random nasoseptal flap for revision skull base reconstruction. J Clin Neurosci :
Shojaeilangari, Seyedehsamaneh; Schmidtlein, C Ross; Rahmim, Arman et al. (2018) Recovery of missing data in partial geometry PET scanners: Compensation in projection space vs image space. Med Phys 45:5437-5449
Sayeed, Sadia; Xing, Deyin; Jenkins, Sarah M et al. (2018) Criteria for Risk Stratification of Vulvar and Vaginal Smooth Muscle Tumors: An Evaluation of 71 Cases Comparing Proposed Classification Systems. Am J Surg Pathol 42:84-94
Han, SoHyun; Stoyanova, Radka; Lee, Hansol et al. (2018) Automation of pattern recognition analysis of dynamic contrast-enhanced MRI data to characterize intratumoral vascular heterogeneity. Magn Reson Med 79:1736-1744
Cerfolio, Robert J; Ghanim, Asem F; Dylewski, Mark et al. (2018) The long-term survival of robotic lobectomy for non-small cell lung cancer: A multi-institutional study. J Thorac Cardiovasc Surg 155:778-786

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