The mission of the Molecular Cytology Core is to deliver cutting-edge, specialized services in support of research projects at the Center. The methodologies employed by the Core allow for the detection in situ (in cells, tissue sections, organoids and whole mounts) of biomarkers (proteins, glycoproteins, lipids, nucleic acids) and molecular processess in normal and pathological conditions. The precise localization of the biomarkers is achieved by optical imaging of fixed and live samples. Several molecular markers (up to seven) can be vizualized simultanously or sequentially in the same sample by multiplex staining, using machine-based protocols. In collaboration with its users, the Core performs comprehensive antibody validations. The Core trains investigators in basic staining principles and assists them in performing manual experiments. The optical imaging modalities within the Core allow for wide field imaging and confocal imaging of tissue sections, thick and cleared tissue samples, cells, live embryos, tumors in live mice, etc. Super-resolution imaging is performed as well as confocal imaging in the IR range to visualize nanomaterials and drugs. With the newly acquired module for fluorescence correlation spectroscopy (FCS) imaging, researchers can study the dynamic behavior of biomolecules. The Core scans user slides with four digital slide scanners, one of which has confocal capabilities. The scanning generates large volumes of data and serves as a valuable resource for image analysis. Microscopy staff provides strong support for the users with 2D and 3D image analysis. The Core?s Atomic Force microscope is heavily used to study molecular interactions with nanometer resolution, to evaluate the effect of inhibitors on pathologic processes, and to assess stiffness of tissues from normal and cancer- bearing animals as well as tissues from cancer patients.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA008748-55
Application #
10084814
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
1997-01-20
Project End
2023-12-31
Budget Start
2021-01-01
Budget End
2021-12-31
Support Year
55
Fiscal Year
2021
Total Cost
Indirect Cost
Name
Sloan-Kettering Institute for Cancer Research
Department
Type
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10065
Assel, Melissa J; Li, Fan; Wang, Ying et al. (2018) Genetic Polymorphisms of CFH and ARMS2 Do Not Predict Response to Antioxidants and Zinc in Patients with Age-Related Macular Degeneration: Independent Statistical Evaluations of Data from the Age-Related Eye Disease Study. Ophthalmology 125:391-397
Rebbeck, Timothy R (see original citation for additional authors) (2018) Mutational spectrum in a worldwide study of 29,700 families with BRCA1 or BRCA2 mutations. Hum Mutat 39:593-620
Yeung, K Simon; Hernandez, Marisol; Mao, Jun J et al. (2018) Herbal medicine for depression and anxiety: A systematic review with assessment of potential psycho-oncologic relevance. Phytother Res 32:865-891
Audenet, Fran├žois; Vertosick, Emily A; Fine, Samson W et al. (2018) Biopsy Core Features are Poor Predictors of Adverse Pathology in Men with Grade Group 1 Prostate Cancer. J Urol 199:961-968
Petrovic, Ivana; Rosen, Evan B; Matros, Evan et al. (2018) Oral rehabilitation of the cancer patient: A formidable challenge. J Surg Oncol 117:1729-1735
Balakrishnan, Mridula; Baylies, Mary K (2018) Myonuclear Positioning and Aneurysms Are LINC'd by Ariande. Dev Cell 45:149-150
McKnight, Brooke N; Kuda-Wedagedara, Akhila N W; Sevak, Kuntal K et al. (2018) Imaging EGFR and HER3 through 89Zr-labeled MEHD7945A (Duligotuzumab). Sci Rep 8:9043
Thor, Maria; Jackson, Andrew; Zelefsky, Michael J et al. (2018) Inter-institutional analysis demonstrates the importance of lower than previously anticipated dose regions to prevent late rectal bleeding following prostate radiotherapy. Radiother Oncol 127:88-95
Wiener, Lori; Rosenberg, Abby R; Lichtenthal, Wendy G et al. (2018) Personalized and yet standardized: An informed approach to the integration of bereavement care in pediatric oncology settings. Palliat Support Care 16:706-711
Damaskos, Penny; Amaya, Beau; Gordon, RuthAnn et al. (2018) Intersectionality and the LGBT Cancer Patient. Semin Oncol Nurs 34:30-36

Showing the most recent 10 out of 8799 publications