The goal of the Flow Cytometry Shared Resource (FCSR) is to provide Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) members with ready access to the necessary expertise and state-of-the-art flow cytometry instrumentation that can be applied to a wide range of cancer biology research. Flow cytometry is a critical technology that provides rapid single-cell analysis and cell sorting through the simultaneous detection of light scatter properties and the presence of multiple cellular molecules or metabolites in or on an individual cell. In addition, the FCSR seeks to foster the development of protocols that can utilize the continually increasing number of available markers and methodologies. The FCSR is focused on addressing the needs of both seasoned flow cytometry users as well as those just discovering the capabilities of flow cytometry technology. To this end, monthly training for the instruments is provided for new users as well as support in experimental design and data interpretation following training. Instruments available include a BD FACS Aria cell sorter (three lasers and eleven parameters), a BD FACS Canto II analyzer (three lasers and ten parameters) and BD FACS Calibur and Accuri analyzers (two lasers and six parameters). In addition to the software with each flow cytometer, additional analysis software is available to analyze multiparameter data to identify and characterize cell subpopulations. DNA histogram analysis software programs are also available for researchers requiring advanced cell cycle analysis. The FCSR is under the direction of Martha Alexander-Miller, Ph.D., and has a staff of 1.33 FTEs. To accomplish the goals of the facility, the FCSR has the following Specific Aims to: 1) Provide cutting-edge analysis instrumentation for flow cytometric analysis; 2) Provide multi-parameter cell sorting for user-defined populations; 3) Provide instrument training for new and existing users; and 4) Provide expertise for experimental design and interpretation of flow cytometry data. In the November 2014 ? October 2015 grant year, the FCSR provided services to forty-two users, 74% of whom were WFBCCC members; of those, 64% had peer-reviewed funding. WFBCCC members accounted for 85% of the service hours.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Wake Forest University Health Sciences
United States
Zip Code
Melvin, Ryan L; Xiao, Jiajie; Berenhaut, Kenneth S et al. (2018) Using correlated motions to determine sufficient sampling times for molecular dynamics. Phys Rev E 98:023307
Bhatt, Nikunj B; Pandya, Darpan N; Dezarn, William A et al. (2018) Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes. Methods Mol Biol 1790:197-208
Gesell, Sabina B; Golden, Shannon L; Limkakeng Jr, Alexander T et al. (2018) Implementation of the HEART Pathway: Using the Consolidated Framework for Implementation Research. Crit Pathw Cardiol 17:191-200
Mao, Chengqiong; Qu, Ping; Miley, Michael J et al. (2018) P-glycoprotein targeted photodynamic therapy of chemoresistant tumors using recombinant Fab fragment conjugates. Biomater Sci 6:3063-3074
Bhatt, Nikunj B; Pandya, Darpan N; Rideout-Danner, Stephanie et al. (2018) A comprehensively revised strategy that improves the specific activity and long-term stability of clinically relevant 89Zr-immuno-PET agents. Dalton Trans 47:13214-13221
Andrews, Rachel N; Caudell, David L; Metheny-Barlow, Linda J et al. (2018) Fibronectin Produced by Cerebral Endothelial and Vascular Smooth Muscle Cells Contributes to Perivascular Extracellular Matrix in Late-Delayed Radiation-Induced Brain Injury. Radiat Res 190:361-373
Zhao, Yan; Li, Fang; Mao, Chengqiong et al. (2018) Multiarm Nanoconjugates for Cancer Cell-Targeted Delivery of Photosensitizers. Mol Pharm 15:2559-2569
Samykutty, Abhilash; Grizzle, William E; Fouts, Benjamin L et al. (2018) Optoacoustic imaging identifies ovarian cancer using a microenvironment targeted theranostic wormhole mesoporous silica nanoparticle. Biomaterials 182:114-126
Xiao, Jiajie; Melvin, Ryan L; Salsbury Jr, Freddie R (2018) Probing light chain mutation effects on thrombin via molecular dynamics simulations and machine learning. J Biomol Struct Dyn :1-18
Mao, Chengqiong; Zhao, Yan; Li, Fang et al. (2018) P-glycoprotein targeted and near-infrared light-guided depletion of chemoresistant tumors. J Control Release 286:289-300

Showing the most recent 10 out of 548 publications