The AECC DMA Sequencing Shared Resource was established in 1992 and has evolved considerably sincethen and, in particular, over the last funding period, to meet the evolving scientific needs of AECC membersand programs. The new name of this facility, Genomics Shared Resource, reflects this expansion of servicesand technologies. The facility now offers, in addition to DMA sequencing, fragment analysis, dHPLC forSNP/mutation discovery, Pyrosequencing SNP typing, pyrosequencing quantitative CpG methylation, andTaqman SNP typing. Real-time PCR is now provided as well. At the time of the last CCSG review, thisFacility provided Affymetrix services, and there was a separate cDNA printing facility. With the furtherdevelopment of microarray technologies, there has been a shift of emphasis to commercially availablemicroarrays. To meet the needs for epigenetic studies, services were contracted with NimbleGen Direct toperform, on their site, gene expression, CGH, chromatin immunoprecipitation on microarrays (ChlP-chip),and a novel genome-wide cytosine methylation assay developed at AECC. NimbleGen services aremanaged by this facility. Within the context of this application all of the microarray services (Affymetrix,NimbleGen and in-house printing) have now been consolidated in this shared resource to provide a singlesource of intellectual guidance to users and enhance cost-effectiveness. The facility provides access to threecomputer workstations with design and analysis software to support a number of the services. Over the pastfive years the capacity of the facility has been increased so that turnaround time has been substantiallydecreased and fees to users have been decreased.
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