The Histopathology Shared Resource provides AECC investigators with a full range of histotechnology and histopathology services with a major focus on mouse models of human cancers. The facility also supports the veterinary services of the AECC Animal Barrier Shared Resource where AECC investigators'mice are housed. The facility provides necropsy and histopathology services for phenotypic analysis along with a full range of histological services including tissue preparation, paraffin and frozen embedding, standard and specialized stains and immunohistochemical analyses to meet the needs of AECC investigators. Several new techniques have been added since the last CCSG review. These include: (1) Staining with the new 'polymer'technology which negates the need for avidin/biotin based staining giving less background staining and reducing protocol length. (2) Immunohistochemical characterization of multiple proliferation markers in the mouse (BrdU, Ki67, PCNA, and pRb) that allows the investigator to choose different proliferative windows, with or without special experimental manipulation prior to sacrifice. (3) Immunohistochemical detection of LacZ protein, which is more sensitive and specific than X-gal staining, and allows integration of this marker into standard paraffin-section based histology. (4) Immunoalkaline phosphatase detection with a new fuchsin substrate that allows xylene-based mounting and higher resolution than aqueous mounting. (5) Double antibody detection combining immunoperoxidase with DAB-nickel detection, and immunoalkaline phosphates with new fuchsin detection. (6) Immunofluorescence protocols for conventional paraffin sections, utilizing fluors with spectra that do not overlap with tissue autofluorescence, and blocking agents that quench autofluorescence. This allows immunohistochemical and conventional light microscopic analyses to be carried out on consecutive sections of routinely processed tissue specimens. A Chemicon Tissue Arrayer was recently acquired for the production of custom tissue arrays. The facility provides consultative support and training to members, their trainees, and their staff. The facility faculty leader, Dr. Rani Sellers, and director, Dr. Radma Mahmood are highly experienced in histotechnology and histopathology with a broad capability in the characterization of mouse models and developmental defects associated with the genetic changes that are induced in animals.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA013330-38
Application #
8113370
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2010-07-01
Budget End
2011-06-30
Support Year
38
Fiscal Year
2010
Total Cost
$189,817
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Li, Ke; Baker, Nicholas E (2018) Regulation of the Drosophila ID protein Extra macrochaetae by proneural dimerization partners. Elife 7:
Xie, Xianhong; Xue, Xiaonan; Strickler, Howard D (2018) Generalized linear mixed model for binary outcomes when covariates are subject to measurement errors and detection limits. Stat Med 37:119-136
Carvajal, Luis A; Neriah, Daniela Ben; Senecal, Adrien et al. (2018) Dual inhibition of MDMX and MDM2 as a therapeutic strategy in leukemia. Sci Transl Med 10:
Boku, S; Izumi, T; Abe, S et al. (2018) Copy number elevation of 22q11.2 genes arrests the developmental maturation of working memory capacity and adult hippocampal neurogenesis. Mol Psychiatry 23:985-992
Mendez-Dorantes, Carlos; Bhargava, Ragini; Stark, Jeremy M (2018) Repeat-mediated deletions can be induced by a chromosomal break far from a repeat, but multiple pathways suppress such rearrangements. Genes Dev 32:524-536
Schloss, Jennifer; Ali, Riyasat; Racine, Jeremy J et al. (2018) HLA-B*39:06 Efficiently Mediates Type 1 Diabetes in a Mouse Model Incorporating Reduced Thymic Insulin Expression. J Immunol 200:3353-3363
Kushnir, Alexander; Santulli, Gaetano; Reiken, Steven R et al. (2018) Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure. Circulation 138:1144-1154
Van Arsdale, Anne R; Arend, Rebecca C; Cossio, Maria J et al. (2018) Insulin-like growth factor 2: a poor prognostic biomarker linked to racial disparity in women with uterine carcinosarcoma. Cancer Med 7:616-625
Ruiz, Penelope D; Gamble, Matthew J (2018) MacroH2A1 chromatin specification requires its docking domain and acetylation of H2B lysine 20. Nat Commun 9:5143
Rohan, Thomas; Ye, Kenny; Wang, Yihong et al. (2018) MicroRNA expression in benign breast tissue and risk of subsequent invasive breast cancer. PLoS One 13:e0191814

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