The transgenic shared resource was founded in 1988 to train and assist AECC investigators in the production and characterization of transgenic mouse strains. This technology allows analysis of gene function through the introduction of DMA sequences into the germ line, usually, although not exclusively, by pronuclear injection of fertilized oocytes. The shared resource supervisor director consults with individual investigators and advises on transgene construct, as well as making plasmids and sequence cassettes available, as well as other reagents. This results in DMA constructs suitable for expression in the mouse. Usually this is in the form of a plasmid but we also offer the injection of BAG DMA. The shared resource prepares the DNA and introduces this DNA into the oocytes by micro-injection. In some cases the sequence of interest is introduced into the oocyte by lentivirus infection. After the manipulation of the oocyte they are transferred to pseudo-pregnant mothers and following birth, the pups are cared for to weaning. At this point mice are returned to the investigator for further analysis. The production of useful transgenics usually takes less than seven weeks from submission of the DNA. We guarantee at least three founders per construct although generally more are generated. We have had essentially 100% success rate with little need for reinjection of constructs, Between 18-25 investigators use this service per year with approximately 100 constructs injected. In addition, the core provides embryo and sperm freezing for long-term storage of mouse lines, in vitro fertilization (IVF) and intra-cytoplasmic sperm injections (ICSI) to rescue frozen samples or difficult to mate strains. We also provide blastomere fusion to generate tetraploid embryos for aggregation with ES cells to determine whether the phenotypes of mutations are intrinsic to the embryo or are the result of placenta! abnormalities.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA013330-39
Application #
8294860
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
2013-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
39
Fiscal Year
2011
Total Cost
$248,050
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Agalliu, Ilir; Chen, Zigui; Wang, Tao et al. (2018) Oral Alpha, Beta, and Gamma HPV Types and Risk of Incident Esophageal Cancer. Cancer Epidemiol Biomarkers Prev 27:1168-1175
Bhargava, Ragini; Sandhu, Manbir; Muk, Sanychen et al. (2018) C-NHEJ without indels is robust and requires synergistic function of distinct XLF domains. Nat Commun 9:2484
Collu, Giovanna M; Jenny, Andreas; Gaengel, Konstantin et al. (2018) Prickle is phosphorylated by Nemo and targeted for degradation to maintain Prickle/Spiny-legs isoform balance during planar cell polarity establishment. PLoS Genet 14:e1007391
Doyle, Christopher R; Moon, Jee-Young; Daily, Johanna P et al. (2018) A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice. Infect Immun 86:
Anayannis, Nicole V; Schlecht, Nicolas F; Ben-Dayan, Miriam et al. (2018) Association of an intact E2 gene with higher HPV viral load, higher viral oncogene expression, and improved clinical outcome in HPV16 positive head and neck squamous cell carcinoma. PLoS One 13:e0191581
Stepankova, Martina; Bartonkova, Iveta; Jiskrova, Eva et al. (2018) Methylindoles and Methoxyindoles are Agonists and Antagonists of Human Aryl Hydrocarbon Receptor. Mol Pharmacol 93:631-644
Maggi, Elaine C; Gravina, Silvia; Cheng, Haiying et al. (2018) Development of a Method to Implement Whole-Genome Bisulfite Sequencing of cfDNA from Cancer Patients and a Mouse Tumor Model. Front Genet 9:6
Ingram, Jessica R; Blomberg, Olga S; Rashidian, Mohammad et al. (2018) Anti-CTLA-4 therapy requires an Fc domain for efficacy. Proc Natl Acad Sci U S A 115:3912-3917
Dulyaninova, Natalya G; Ruiz, Penelope D; Gamble, Matthew J et al. (2018) S100A4 regulates macrophage invasion by distinct myosin-dependent and myosin-independent mechanisms. Mol Biol Cell 29:632-642
Chen, Zigui; Schiffman, Mark; Herrero, Rolando et al. (2018) Classification and evolution of human papillomavirus genome variants: Alpha-5 (HPV26, 51, 69, 82), Alpha-6 (HPV30, 53, 56, 66), Alpha-11 (HPV34, 73), Alpha-13 (HPV54) and Alpha-3 (HPV61). Virology 516:86-101

Showing the most recent 10 out of 1508 publications