Imaging plays a vital role in the research programs of many AECC members. The Analytical Imaging Facility (AIF) makes routine and complex imaging technologies available to the entire AECC community. Microscope technology ranges from traditional brightfield, to epi-fluorescence, to laser scanning confocal, to high resolution transmission and scanning electron microscopy including cryoEM of macromolecules in this truly comprehensive shared resource. This wide range of imaging modalities allows for the study of animal models of disease. Cells expressing or labeled with fluorescent reporter molecules may be imaged in the whole animal, whole organ, single cell or cell compartments. Live cells may be imaged in real time to monitor physiological processes. Photo-activatable fluorescence molecules may be manipulated by light for dynamic in vivo studies. Confocal microscopes and epi-fluorescence paired with deconvolution enables 3D imaging of fixed material or 4D imaging of live cells. Traditional ultrastructure analysis by transmission electron microscopy is expanded to 3D by electron tomography. The recent installation of a field emission scanning electron microscope (SEM) has expanded the facility's SEM capabilities to include automated large area mapping, 3D image reconstruction, x-ray microanalysis, correlative fluorescence and SEM imaging and imaging frozen hydrated samples. AIF staff assist users in experimental design, data collection, quantitative image analysis and presentation. New users are taught imaging techniques required for their specific research objective, while experienced users may customize any imaging station, utilizing the facility's large inventory of optics and accessories. The facility offers customized full service sample preparation for electron microscopy, to ensure quality control and to offer the widest range of techniques. The AIF staff has the expertise to prepare samples by methods that include chemical fixation, embedding in resin, ultrathin sectioning, immunogold labeling and negative staining. The facility offers a full range of low temperature techniques for electron microscopy including quick freezing by plunge, metal mirror or high pressure freezing. Frozen samples can undergo freeze-substitution, freeze fracture, rotary shadow or cryosectioning and are then imaged in the TEM or SEM at ambient or low temperature. The AIF provides support for the microscopy requirements of the Molecular Cytogenetics and Histopathology Shared Resources. Since the last CCSG review, important new additions of equipment, personnel and services have been made, which have substantially enhanced the imaging capabilities at AECC.
The Analytical Imaging Facility provides microscope imaging technologies and quantitative image analysis supporting the translational research mission and goals of the Albert Einstein Cancer Center (AECC). As an NCI-designated Cancer Center, AECC contributes to the national effort to reduce morbidity and mortality from cancer.
|Vilchèze, Catherine; Copeland, Jacqueline; Keiser, Tracy L et al. (2018) Rational Design of Biosafety Level 2-Approved, Multidrug-Resistant Strains of Mycobacterium tuberculosis through Nutrient Auxotrophy. MBio 9:|
|Cabahug-Zuckerman, Pamela; Stout Jr, Randy F; Majeska, Robert J et al. (2018) Potential role for a specialized ?3 integrin-based structure on osteocyte processes in bone mechanosensation. J Orthop Res 36:642-652|
|Yu, Bo; Davidson, Nancy E (2018) Gonadotropin-Releasing Hormone (GnRH) Agonists for Fertility Preservation: Is POEMS the Final Verse? J Natl Cancer Inst :|
|Wang, Xiaohua; Duan, Zhi; Yu, Guojun et al. (2018) Human Immunodeficiency Virus Tat Protein Aids V Region Somatic Hypermutation in Human B Cells. MBio 9:|
|Gradissimo, Ana; Lam, Jessica; Attonito, John D et al. (2018) Methylation of High-Risk Human Papillomavirus Genomes Are Associated with Cervical Precancer in HIV-Positive Women. Cancer Epidemiol Biomarkers Prev 27:1407-1415|
|Mirabello, Lisa; Clarke, Megan A; Nelson, Chase W et al. (2018) The Intersection of HPV Epidemiology, Genomics and Mechanistic Studies of HPV-Mediated Carcinogenesis. Viruses 10:|
|Drelon, Coralie; Belalcazar, Helen M; Secombe, Julie (2018) The Histone Demethylase KDM5 Is Essential for Larval Growth in Drosophila. Genetics 209:773-787|
|Hudgins, Adam D; Tazearslan, Cagdas; Tare, Archana et al. (2018) Age- and Tissue-Specific Expression of Senescence Biomarkers in Mice. Front Genet 9:59|
|Willis, Ian M; Moir, Robyn D; Hernandez, Nouria (2018) Metabolic programming a lean phenotype by deregulation of RNA polymerase III. Proc Natl Acad Sci U S A 115:12182-12187|
|Hayama, Ryo; Sparks, Samuel; Hecht, Lee M et al. (2018) Thermodynamic characterization of the multivalent interactions underlying rapid and selective translocation through the nuclear pore complex. J Biol Chem 293:4555-4563|
Showing the most recent 10 out of 1508 publications