A fully Cancer Center-managed facility, the Genomics Technologies Shared Resource consists of theMicroarray and Molecular Cytogenetics facilities which provide access to specialized instrumentation andtechnical expertise in high-throughput genomic technologies and molecular cytogenetics for use inindividual and collaborative research programs. Services provided by the facility are: Microarray based genome-wide analysis of gene expression, genetic markers, and DNA copynumber aberrations Microarray based genome-wide analysis of chromatin modifications Validation of microarray and other data by high-throughput quantitative PCR Molecular cytogenetic analysis by fluorescence in situ hybridization (FISH) and spectral karyotyping(SKY) Evaluation and incorporation of emerging genomic technologies and promoting their integration intoresearch programs of HICCC investigators Investigator education and advice in adopting genome wide profiling and molecular cytogenetics intheir cancer research.The microarray component of the Genomics Technologies Shared Resource thus provides acomprehensive set of integrated genomic analysis tools and services that facilitate the high throughputanalysis of genetic and epigenetic alterations in human cancer in basic and translational researchprograms. These services, which are currently focused on procedures with several different microarrayplatforms from Affymetrix and Agilent, with ancillary methods including real-time PCR andPhosphorimaging, are closely linked to those provided by the Biomedical Informatics Shared Resource.The molecular cytogenetics facility provides SKY and FISH characterization of human and mouse tumorcells with efficient turnaround times utilizing state-of-the-art technologies. Together these facilities andservices provide an integrated platform for basic and translational genomics research in cancer biology atthe HICCC. Future plans include evaluating and potentially incorporating an ultra-high-throughputsequencing service into this Resource. During the last period of the CCSG, 53% of the investigators usingthe facility were Cancer Center members with peer-reviewed funding with those members representingfrom 50% to 98% of the usage of the available services. The proposed total operating budget of the facilityis $392,489, of which we are requesting $ 66,831 from the CCSG.
Showing the most recent 10 out of 331 publications
