Transgenic and knockout technologies are an extremely powerful set of tools used increasingly to investigate normal development and physiological processes, as well as a variety of disease processes, including cancer. Rapid progress in molecular epidemiology and genomic approaches to cancer now promise an explosive increase in the rate of identification of genetic loci associated with cancer. Any understanding of how such loci function in normal and pathological processes will depend heavily on experiments in genetically altered mice. The Transgenic Core Facility was established in April 1993 with Developmental Funds from the Cancer Center Support Grant. The primary services provided by the technical staff of the Transgenic Core Facility are the production of transgenic mice by pro-nuclear injection and the production of knockout by ES cell injection into blastocysts. The Core can also assist with genotyping of mice, and proposes to expand this service in a joint venture with the Genomics Core Facility. Finally, the Core provides advice and expert consultation on vector preparation, mouse developmental biology, and methods and approaches to testing gene function in mice. Dr. Robert Maxson, Professor of Biochemistry and Molecular Biology, directs the Transgenic Core Facility and has extensive experience with transgenic and knockout mice. The facility is staffed by Frank Sangiorgi, Ph.D., who manages the facility. Dr. Nancy Wu. Who performs most of the DNA injections and egg manipulations and Ms. Youzhen Yan, who monitors the mice and performs various technical services. The facility is located on the 7th floor of the Norris building in Cancer Center Vivarium space and consists of a microinjection room and two dedicated mouse holding rooms.

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National Cancer Institute (NCI)
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Poulard, Coralie; Baulu, Estelle; Lee, Brian H et al. (2018) Increasing G9a automethylation sensitizes B acute lymphoblastic leukemia cells to glucocorticoid-induced death. Cell Death Dis 9:1038
Lang, Julie E; Brownson, Kirstyn E (2018) ASO Author Reflections: The Whole Transcriptome Landscape of Circulating Tumor Cells in Nonmetastatic Breast Cancer. Ann Surg Oncol :
Milam, Joel; Slaughter, Rhona; Tobin, Jessica L et al. (2018) Childhood Cancer Survivorship and Substance Use Behaviors: A Matched Case-Control Study Among Hispanic Adolescents and Young Adults. J Adolesc Health 63:115-117
Guo, Yu; Perez, Andrew A; Hazelett, Dennis J et al. (2018) CRISPR-mediated deletion of prostate cancer risk-associated CTCF loop anchors identifies repressive chromatin loops. Genome Biol 19:160
Suenaga, Mitsukuni; Schirripa, Marta; Cao, Shu et al. (2018) Potential role of PIN1 genotypes in predicting benefit from oxaliplatin-based and irinotecan-based treatment in patients with metastatic colorectal cancer. Pharmacogenomics J 18:623-632
Singh, Hardeep P; Wang, Sijia; Stachelek, Kevin et al. (2018) Developmental stage-specific proliferation and retinoblastoma genesis in RB-deficient human but not mouse cone precursors. Proc Natl Acad Sci U S A 115:E9391-E9400
Tsai, Yuan-Li; Ha, Dat P; Zhao, He et al. (2018) Endoplasmic reticulum stress activates SRC, relocating chaperones to the cell surface where GRP78/CD109 blocks TGF-? signaling. Proc Natl Acad Sci U S A 115:E4245-E4254
Rodrigues, Luana L S; Morgado, Mariza G; Sahasrabuddhe, Vikrant V et al. (2018) Cervico-vaginal self-collection in HIV-infected and uninfected women from Tapajós region, Amazon, Brazil: High acceptability, hrHPV diversity and risk factors. Gynecol Oncol 151:102-110
Valentin-Torres, Alice; Savarin, Carine; Barnett, Joslyn et al. (2018) Blockade of sustained tumor necrosis factor in a transgenic model of progressive autoimmune encephalomyelitis limits oligodendrocyte apoptosis and promotes oligodendrocyte maturation. J Neuroinflammation 15:121
Ricker, Charité N; Koff, Rachel B; Qu, Chenxu et al. (2018) Patient communication of cancer genetic test results in a diverse population. Transl Behav Med 8:85-94

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