The Molecular Genomics Core (MGC) is a recent fusion of the DNA Core (formerly the Microchemical Core, founded in 1984), the Genomics Core founded in 1999 and the Microarray Core founded in 1998. This consolidation will ensure that there is no duplication in the acquisition of expensive state-of-the-art equipment and that the resource functions in an efficient and seamless manner to provide optimized service to investigators. The merger reduced redundancies in services, provided a single structure, which allowed the Core to adjust to changing demands over time and facilitated the interaction of shared expertise. MGC services are broadly divided into two main categories - sample preparation and analytical assays. Biospecimen processing is available for research and clinical samples from body fluids, fresh and archival tissue, and non-mammalian sources. The assays available in the MGC are based on the ability to identify and interrogate genetic (DNA profiling) and epigenetic (epigenetic profiling) variation. Additionally, expression profiling encompassing both genetic and epigenetic components is provided, including direct changes to DNA sequences effecting gene expression levels and DNA methylation and histone modifications playing roles in modified gene expression levels through changes in chromatin structure. The MGC has already provided services (annual total budget of >$6.5 million) to more than 175 USC Norris Comprehensive Cancer Center (NCCC) members at different levels of throughput, including single base resolution of the entire genome and single base interrogation of DNA, RNA and chromatin.

Public Health Relevance

MGC services are faster and cheaper than commercial operations and are sometimes even unobtainable from such sources. Furthermore, researchers can seek advice in the design of studies and instruction in the preparation of samples that accelerate the successful execution of experiments. The ready availability of these technologies enables NCCC members, whether basic, population science or clinical researchers, to rapidly bring the power of molecular biology to bear on attacking the fundamental problems of cancer, as well as translating these discoveries for use in the clinic.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014089-39
Application #
8589348
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
39
Fiscal Year
2014
Total Cost
$371,387
Indirect Cost
$135,142
Name
University of Southern California
Department
Type
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Suenaga, Mitsukuni; Schirripa, Marta; Cao, Shu et al. (2018) Potential role of PIN1 genotypes in predicting benefit from oxaliplatin-based and irinotecan-based treatment in patients with metastatic colorectal cancer. Pharmacogenomics J 18:623-632
Singh, Hardeep P; Wang, Sijia; Stachelek, Kevin et al. (2018) Developmental stage-specific proliferation and retinoblastoma genesis in RB-deficient human but not mouse cone precursors. Proc Natl Acad Sci U S A 115:E9391-E9400
Tsai, Yuan-Li; Ha, Dat P; Zhao, He et al. (2018) Endoplasmic reticulum stress activates SRC, relocating chaperones to the cell surface where GRP78/CD109 blocks TGF-? signaling. Proc Natl Acad Sci U S A 115:E4245-E4254
Rodrigues, Luana L S; Morgado, Mariza G; Sahasrabuddhe, Vikrant V et al. (2018) Cervico-vaginal self-collection in HIV-infected and uninfected women from Tapajós region, Amazon, Brazil: High acceptability, hrHPV diversity and risk factors. Gynecol Oncol 151:102-110
Valentin-Torres, Alice; Savarin, Carine; Barnett, Joslyn et al. (2018) Blockade of sustained tumor necrosis factor in a transgenic model of progressive autoimmune encephalomyelitis limits oligodendrocyte apoptosis and promotes oligodendrocyte maturation. J Neuroinflammation 15:121
Ricker, Charité N; Koff, Rachel B; Qu, Chenxu et al. (2018) Patient communication of cancer genetic test results in a diverse population. Transl Behav Med 8:85-94
Jayne, Jordanna G; Bensman, Timothy J; Schaal, Justin B et al. (2018) Rhesus ?-Defensin-1 Attenuates Endotoxin-induced Acute Lung Injury by Inhibiting Proinflammatory Cytokines and Neutrophil Recruitment. Am J Respir Cell Mol Biol 58:310-319
Tobin, Jessica; Miller, Kimberly A; Baezconde-Garbanati, Lourdes et al. (2018) Acculturation, Mental Health, and Quality of Life among Hispanic Childhood Cancer Survivors: A Latent Class Analysis. Ethn Dis 28:55-60
Harris, Holly R; Babic, Ana; Webb, Penelope M et al. (2018) Polycystic Ovary Syndrome, Oligomenorrhea, and Risk of Ovarian Cancer Histotypes: Evidence from the Ovarian Cancer Association Consortium. Cancer Epidemiol Biomarkers Prev 27:174-182
Lee, Brian H; Stallcup, Michael R (2018) Different chromatin and DNA sequence characteristics define glucocorticoid receptor binding sites that are blocked or not blocked by coregulator Hic-5. PLoS One 13:e0196965

Showing the most recent 10 out of 842 publications